Anti-HDAC4 + 5 + 9 antibody (ab131524)
Key features and details
- Rabbit polyclonal to HDAC4 + 5 + 9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC4 + 5 + 9 antibody
See all HDAC4 + 5 + 9 primary antibodies -
Description
Rabbit polyclonal to HDAC4 + 5 + 9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to amino acids around 244-248/257-261/218-222 of Human HDAC4 + 5 + 9 (NP_006028.2 NP_001015053.1 NP_055522.1)
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Positive control
- Methanol-fixed HeLa cells, 293 and HeLa cell lysates, Human lung carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 119, 122, 111 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 119, 122, 111 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Relevance
HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. HDAC5: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. HDAC9: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. -
Cellular localization
HDAC4: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation. HDAC5: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1. HDAC9: Nucleus -
Database links
- Entrez Gene: 10014 Human
- Entrez Gene: 9734 Human
- Entrez Gene: 9759 Human
- Entrez Gene: 15184 Mouse
- Entrez Gene: 208727 Mouse
- Entrez Gene: 79221 Mouse
- Omim: 605314 Human
- Omim: 605315 Human
see all -
Alternative names
- AHO3 antibody
- Antigen NY-CO-9 antibody
- BDMR antibody
see all
Images
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All lanes : Anti-HDAC4 + 5 + 9 antibody (ab131524) at 1/500 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Predicted band size: 119, 122, 111 kDa -
Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131524 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelled with ab131524 at 1/50 dilution (left) or the same antibody preincubated with blocking peptide (right).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131524 has been referenced in 1 publication.
- Aboukhatwa SM et al. Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue. ChemMedChem 14:1096-1107 (2019). PubMed: 30921497