|
|
|
|
Immunocytochemistry/ Immunofluorescence - HDAC4 antibody - ChIP Grade (ab1437)
(enlarge)
|
|
|
|
|
Product Name
|
HDAC4 antibody - ChIP Grade
|
| |
See all HDAC4 antibodies (14)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to HDAC4 - ChIP Grade
|
|
Immunogen
|
This information is considered to be commercially sensitive(Peptide available as ab53331.)
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
The antibody detects ~140 kDa histone deacetylase 4. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 5, 6, 7, and 8.
|
|
Tested applications
(see key)
|
ChIP, ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use 1µl for 105 cells.
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic and Nuclear. Shuttles between the nucleus and the cytoplasm.
|
|
Research areas
|
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
|
|
Relevance
|
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.
HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.
|
|
|
HDAC4 antibody - ChIP Grade (ab1437) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC4 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for HDAC4 antibody - ChIP Grade images, references, products related to ab1437 and other tools.
|
| |
HDAC4 antibody - ChIP Grade images:
|
 Immunocytochemistry/ Immunofluorescence - HDAC4 antibody - ChIP Grade (ab1437)
ICC/IF image of ab1437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1437, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
References for HDAC4 antibody - ChIP Grade (ab1437)
|
|
This product has been used in: (two most recent references)
Gomez-Duran A et al. Recruitment of CREB1 and histone deacetylase 2 (HDAC2) to the mouse Ltbp-1 promoter regulates its constitutive expression in a dioxin receptor-dependent manner. J Mol Biol 380:1-16 (2008). ChIP; Mouse. PubMed: 18508077
Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). PubMed: 11057899
See all 3 publication references for this product.
If you publish research using ab1437 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to HDAC4
|
| |
HDAC4 antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding HDAC4 antibody - ChIP Grade |
Customer FAQs regarding HDAC4 antibody - ChIP Grade |
Protocols for HDAC4 antibody - ChIP Grade |
Price and availability of products related to HDAC4 antibody - ChIP Grade |
| |
HDAC4 antibody - ChIP Grade - related products:
|
|
|
| |
HDAC4 antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about HDAC4 antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
