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Overview
Product name
Anti-HDAC4 antibody - ChIP Grade
See all HDAC4 products (16) ...
Description
Rabbit polyclonal to HDAC4 - ChIP Grade
Specificity
The antibody detects ~140 kDa histone deacetylase 4. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 5, 6, 7, and 8.
Tested applications
ICC/IF, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
This information is considered to be commercially sensitive(Peptide available as ab53331.)
General notes
Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 119 kDa.Can be blocked with HDAC4 peptide (ab53331).
ChIP: Use 1µl for 105 cells.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Post-translational
modifications
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
Target information above from: UniProt accessionP56524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.5.1.98 antibody
- HA6116 antibody
- HD 4 antibody
see all
Database links
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all
Anti-HDAC4 antibody - ChIP Grade images:
Immunocytochemistry/ Immunofluorescence - HDAC4 antibody - ChIP Grade (ab1437)
ICC/IF image of ab1437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1437, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-HDAC4 antibody - ChIP Grade (ab1437)
All lanes : Anti-HDAC4 antibody - ChIP Grade (ab1437) at 1/500 dilution
Lane 1 : HDAC4 KO whole mouse brain (P3)
Lane 2 : WT whole mouse brain (P3)
Lysates/proteins at 30 µg per lane.
Secondary
Anti-Rabbit IgG, HRP-conjugated F(ab')2 Fragment at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 119 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
References for Anti-HDAC4 antibody - ChIP Grade (ab1437)
This product has been referenced in:
- Kang JSet al. Repression of Runx2 function by TGF-beta through recruitment of class II histone deacetylases by Smad3. EMBO J 24:2543-55 (2005). WB, ChIP; Mouse.Read more (PubMed: 15990875) »
- Kehat Iet al. Modulation of chromatin position and gene expression by HDAC4 interaction with nucleoporins. J Cell Biol 193:21-9 (2011).Read more (PubMed: 21464227) »
See all 5 publications for this product
Publishing research using ab1437? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"