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Overview
Product name
Anti-HDAC4 antibody [HDAC-144] - ChIP Grade
See all HDAC4 products (16) ...
Description
Mouse monoclonal [HDAC-144] to HDAC4 - ChIP Grade
Tested applications
ELISA, ICC, IP, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken
Immunogen
Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine.
MSSQSHPDGL SGRDQPVEL
Positive control
Total cell extracts of NIH3T3 fibroblast cells.
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
HDAC-144
Isotype
IgG2a
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).
ChIP: Use at an assay dependent concentration. (PubMed: 21586557)
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Post-translational
modifications
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
Target information above from: UniProt accessionP56524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.5.1.98 antibody
- HA6116 antibody
- HD 4 antibody
see all
Database links
- Entrez Gene: 374207 Chicken
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P83038 Chicken
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
see all
References for Anti-HDAC4 antibody [HDAC-144] - ChIP Grade (ab12171)
This product has been referenced in:
- Matsuda KIet al. Histone Deacetylation during Brain Development Is Essential for Permanent Masculinization of Sexual Behavior. Endocrinology 152:2760-7 (2011). WB, ChIP; Rat.Read more (PubMed: 21586557) »
- Kozhemyakina Eet al. PTHrP represses chondrocyte hypertrophy through a PP2A/HDAC4/MEF2 pathway. Mol Cell Biol : (2009).Read more (PubMed: 19704004) »
See all 4 publications for this product
Publishing research using ab12171? Please let us know so that we can cite the reference in this datasheet
![HDAC4 antibody [HDAC-144] for Western blot in Mouse (12171)](/ps/datasheet/images/12/ab12171/HDAC4-Primary-antibodies-ab12171-3.jpg)
![HDAC4 antibody [HDAC-144] for WB in Human (12171)](/ps/datasheet/images/12/ab12171/HDAC4-Primary-antibodies-ab12171-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"