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Read our guarantee »Anti-HDAC4 antibody
See all HDAC4 products (16) ...
Rabbit polyclonal to HDAC4
ICC/IF, WB, IP, Electron Microscopymore details
Reacts with
Mouse, Rat, Rabbit, Human
Predicted to work with
Chicken
Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.
MSSQSHPDGL SGRDQPVEL
Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation).
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Polyclonal
IgG
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Our Abpromise guarantee covers the use of ab12172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/250
WB: 1/500 - 1/1000.Predicted molecular weight: 140 kDa.(Additional weak bands may be detected when blotting using different extract preparations.)
IP: Use at an assay dependent dilution. (10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells.)
EM: Use at an assay dependent dilution. (PubMed: 18250163)
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Ubiquitous.
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
Belongs to the histone deacetylase family. HD type 2 subfamily.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
Target information above from: UniProt accessionP56524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - HDAC4 antibody (ab12172)

All lanes : Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 1 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lane 2 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lane 3 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lane 4 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lysates/proteins at 60 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG. at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 minutes
Legend details amount of betulinic acid per lane.
This image is courtesy of an Anonymous Abreview.
Western blot - Anti-HDAC4 antibody (ab12172)

All lanes : Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 1 : Lysate from wt control mouse heart tissue
Lane 2 : Lysate from Gq TG mouse heart tissue
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 140 kDa
Observed band size : 140 kDa
Exposure time : 2 minutes
Image courtesy of an anonymous Abreview.
This product has been referenced in:
See all 3 publications for this product
Publishing research using ab12172? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 1 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lane 2 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lane 3 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lane 4 : Human Bladder cancer cell lysate treated with Betulinic acid, curcumin, Gefitinib for 48 hours.
Lysates/proteins at 60 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG. at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 minutes
Legend details amount of betulinic acid per lane.
This image is courtesy of an Anonymous Abreview.

All lanes : Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 1 : Lysate from wt control mouse heart tissue
Lane 2 : Lysate from Gq TG mouse heart tissue
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 140 kDa
Observed band size : 140 kDa
Exposure time : 2 minutes
Image courtesy of an anonymous Abreview.
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