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Product Name
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HDAC4 antibody
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See all HDAC4 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HDAC4
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Immunogen
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Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.
BLAST 'MSSQSHPDGLSGRDQPVEL' with  or 
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Reacts with
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Hu, Ms, Rat, Rb
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Predicted to react
(species key)
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Chk
(Due to sequence homology)
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Tested applications
(see key)
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EM, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: 1/250, this is determined by indirect immunofluorescence using HEK 293T cells expressing recombinant mouse HDAC4
EM: Use at an assay dependent dilution PMID 18250163.
IP: Use at an assay dependent dilution. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells.
WB: Use at an assay dependent dilution. Predicted molecular weight: 140 kDa. A minimum working dilution of 1/1000 is determined by blotting using whole extracts of mouse NIH-3T3 cells. A minimum working dilution of 1/500 is determined by blotting using whole extracts of rat brain. Additional weak bands may be detected when blotting using different extract preparations. Detection of HDAC4 by blotting is specifically inhibited with the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HDAC Activity Colorimetric Assay Kit (ab1432)
and HDAC Activity Fluorometric Assay Kit(ab1438) are also Available
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Positive control
(see definition)
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Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation).
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Cellular localization
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Cytoplasmic and Nuclear. Shuttles between the nucleus and the cytoplasm.
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
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Relevance
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Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.
HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
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Purification notes
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The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
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Form
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Liquid
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Concentration
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1.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for HDAC4 antibody (ab12172)
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This product has been used in: (two most recent references)
Gupta MP et al. HDAC4 and PCAF Bind to Cardiac Sarcomeres and Play a Role in Regulating Myofilament Contractile Activity. J Biol Chem 283:10135-46 (2008). WB, ICC/IF, EM; Mouse, Rat, Rabbit. PubMed: 18250163
Portal D et al. Epstein-Barr nuclear antigen leader protein coactivates transcription through interaction with histone deacetylase 4. Proc Natl Acad Sci U S A 103:19278-83 (2006). WB PubMed: 17159145
If you publish research using ab12172 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HDAC4
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HDAC4 antibody - more information
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Customer reviews (feedback) regarding HDAC4 antibody |
Customer FAQs regarding HDAC4 antibody |
Protocols for HDAC4 antibody |
Price and availability of products related to HDAC4 antibody |
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Contact Abcam with a Technical Enquiry about HDAC4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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