Overview

  • Product name
  • Description
    Rabbit polyclonal to HDAC4
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-P, Electron Microscopymore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    MSSQSHPDGLSGRDQPVEL

    , corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.

  • Positive control
    • Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation).
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use

Properties

Applications

Our Abpromise guarantee covers the use of ab12172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/500 - 1/1000. Predicted molecular weight: 140 kDa. Additional weak bands may be detected when blotting using different extract preparations.
IP Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells.
IHC-P Use at an assay dependent concentration. PubMed: 21953612
Electron Microscopy Use at an assay dependent concentration. PubMed: 18250163

Target

  • Function
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similarities
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • Domain
    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Images

  • ab12172 staining HDAC4 in human rhabdomyosarcoma (soft tissue sarcoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was enzymatic using a proteinase K buffer for 5 minutes at 37oC. Samples were incubated with primary antibody (1/200) for 25 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Wein MN  et al. SIKs control osteocyte responses to parathyroid hormone. Nat Commun 7:13176 (2016). Mouse . Read more (PubMed: 27759007) »
  • Zeng LS  et al. Overexpressed HDAC4 is associated with poor survival and promotes tumor progression in esophageal carcinoma. Aging (Albany NY) 8:1236-49 (2016). WB . Read more (PubMed: 27295551) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (rhabdomyosarcoma)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K, 5 min, 37C
Permeabilization
No
Specification
rhabdomyosarcoma
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 30 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Sample
Mouse Tissue lysate - whole (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (9% gel)
Sample
Rat Cell lysate - whole cell (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Hippocampus)
Loading amount
15 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seungyeon Ko

Verified customer

Submitted May 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (hippocampus)
Loading amount
25 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Sung-Eun WANG

Verified customer

Submitted Apr 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up