Overview

  • Product nameAnti-HDAC4 antibodySee all HDAC4 primary antibodies ...
  • Description
    Rabbit polyclonal to HDAC4
  • Tested applicationsICC/IF, WB, IP, IHC-P, Electron Microscopymore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    MSSQSHPDGLSGRDQPVEL

    , corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken.

  • Positive control
    • Whole extracts of rat brain, whole extracts of mouse NIH-3T3 cell (Western blotting) and HeLa nuclei (immunoprecipitation).
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use

Properties

Applications

Our Abpromise guarantee covers the use of ab12172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/500 - 1/1000. Predicted molecular weight: 140 kDa. Additional weak bands may be detected when blotting using different extract preparations.
IP Use at an assay dependent concentration. 10-20 µg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells.
IHC-P Use at an assay dependent concentration. PubMed: 21953612
Electron Microscopy Use at an assay dependent concentration. PubMed: 18250163

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHO3 antibody
    • BDMR antibody
    • EC 3.5.1.98 antibody
    • HA6116 antibody
    • HD 4 antibody
    • HD4 antibody
    • HDAC 4 antibody
    • HDAC A antibody
    • HDAC4 antibody
    • HDAC4_HUMAN antibody
    • HDACA antibody
    • Histone Deacetylase 4 antibody
    • Histone Deacetylase A antibody
    • KIAA0288 antibody
    see all

Anti-HDAC4 antibody images

  • All lanes : Anti-HDAC4 antibody (ab12172) at 1/500 dilution

    Lane 1 : Lysate from wt control mouse heart tissue
    Lane 2 : Lysate from Gq TG mouse heart tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 140 kDa
    Observed band size : 140 kDa


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-HDAC4 antibody (ab12172)

This product has been referenced in:
  • Rönn T  et al. A six months exercise intervention influences the genome-wide DNA methylation pattern in human adipose tissue. PLoS Genet 9:e1003572 (2013). WB ; Human . Read more (PubMed: 23825961) »
  • Lenoir O  et al. Specific control of pancreatic endocrine ß- and d-cell mass by class IIa histone deacetylases HDAC4, HDAC5, and HDAC9. Diabetes 60:2861-71 (2011). IHC-P ; Rat . Read more (PubMed: 21953612) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (9% gel)
Sample Rat Cell lysate - whole cell (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 30 2013

Application Western blot
Sample Rat Cell lysate - whole cell (Hippocampus)
Loading amount 15 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10% SDS gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seungyeon Ko

Verified customer

Submitted May 14 2013

Application Western blot
Sample Rabbit Cell lysate - whole cell (hippocampus)
Loading amount 25 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (10%SDS gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Sung-Eun WANG

Verified customer

Submitted Apr 17 2013

Application Western blot
Sample Mouse Tissue lysate - other (MOUSE HEART)
Loading amount 20 µg
Specification MOUSE HEART
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Jul 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"