Anti-HDAC4 antibody (ab79521)
Key features and details
- Rabbit polyclonal to HDAC4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC4 antibody
See all HDAC4 primary antibodies -
Description
Rabbit polyclonal to HDAC4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken, Zebrafish, Orangutan -
Immunogen
Synthetic peptide:
CISSDPRYWYGKTQHS
, corresponding to amino acids 194-209 of Human HDAC4 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml.
|
Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsPhosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. - Information by UniProt
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Database links
- Entrez Gene: 9759 Human
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
- Unigene: 23483 Rat
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Alternative names
- AHO3 antibody
- BDMR antibody
- EC 3.5.1.98 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab79521 has been referenced in 8 publications.
- Xiao L et al. Inhibition of HDAC4 by GSK3ß leads to downregulation of KLF5 and ASK1 and prevents the progression of intravertebral disc degeneration. Clin Epigenetics 13:53 (2021). PubMed: 33691773
- Li YY et al. PTHrP promotes development of mouse preimplantation embryos through the AKT/cyclin D1 pathway and nuclear translocation of HDAC4. J Cell Physiol 236:7001-7013 (2021). PubMed: 33724469
- Guerin MV et al. TGFß blocks IFNa/ß release and tumor rejection in spontaneous mammary tumors. Nat Commun 10:4131 (2019). PubMed: 31511510
- Stoney PN et al. A seasonal switch in histone deacetylase gene expression in the hypothalamus and their capacity to modulate nuclear signaling pathways. Brain Behav Immun 61:340-352 (2017). PubMed: 27993690
- Shen W et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . PubMed: 24923522
- Cui H et al. Eating disorder predisposition is associated with ESRRA and HDAC4 mutations. J Clin Invest 123:4706-13 (2013). PubMed: 24216484
- Takase K et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . PubMed: 23469282
- Funato H et al. Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus. PLoS One 6:e18950 (2011). IHC-FoFr ; Mouse . PubMed: 21526203