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ab39408 has been referenced in 3 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)
Predicted band size : 119 kDa
Predicted band size : 119 kDa
20 μg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit.
Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.
All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Lysate from murine heart tissue 1
Lane 2 : Lysate from murine heart tissue 2
Lysates/proteins at 20 µg per lane.
Secondary
HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 119 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Image courtesy of an anonymous Abreview.
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