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Overview
Product name
Anti-HDAC4 (phospho S632) antibody
See all HDAC4 products (16) ...
Description
Rabbit polyclonal to HDAC4 (phospho S632)
Specificity
ab39408 detects endogenous levels of HDAC4 only when phosphorylated at serine 632.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-SP-S-P)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 119 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Post-translational
modifications
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
Target information above from: UniProt accessionP56524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.5.1.98 antibody
- HA6116 antibody
- HA6116 antibody
see all
Database links
- Entrez Gene: 9759 Human
- Entrez Gene: 208727 Mouse
- Entrez Gene: 363287 Rat
- Omim: 605314 Human
- SwissProt: P56524 Human
- SwissProt: Q6NZM9 Mouse
- SwissProt: Q99P99 Rat
- Unigene: 20516 Human
see all
Anti-HDAC4 (phospho S632) antibody images:
Western blot - HDAC4 (phospho S632) antibody (ab39408)
All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Extract from Jurkat cells (5-30ug of total protein)
Lane 2 : Extract from Jurkat cells treated with Calyculin A (5-30ug of total protein)
Predicted band size : 119 kDa
Western blot - HDAC4 (phospho S632) antibody (ab39408)
Predicted band size : 119 kDa
20 μg of nuclear lysate prepared from mouse liver was diluted in SDS buffer and run on SDS-PAGE gel. Samples were transferred to nitrocellulose overnight, and blocked with 5% BSA for 8 hours. They were then incubated overnight with primary antibodies, which included an anti-HDAC1, 1/1000 and ab39408, 1/1000. After washing, membranes were incubated with horseradish peroxidase-coupled rabbit or mouse secondary antibodies (1/5000 to 1/10,000) in 5% milk for 1 hour and developed with a Super Signal West Pico chemiluminescent kit.
Image from Evankovich J et al, J Biol Chem. 2010 Dec 17;285(51):39888-97. Epub 2010 Oct 11, Fig 4.
Western blot - Anti-HDAC4 (phospho S632) antibody (ab39408)
All lanes : Anti-HDAC4 (phospho S632) antibody (ab39408) at 1/500 dilution
Lane 1 : Lysate from murine heart tissue 1
Lane 2 : Lysate from murine heart tissue 2
Lysates/proteins at 20 µg per lane.
Secondary
HRp conjugated goat anti-rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 119 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Image courtesy of an anonymous Abreview.
References for Anti-HDAC4 (phospho S632) antibody (ab39408)
This product has been referenced in:
- Li Het al. Calmodulin kinase II is required for angiotensin II-mediated vascular smooth muscle hypertrophy. Am J Physiol Heart Circ Physiol 298:H688-98 (2010).Read more (PubMed: 20023119) »
- Evankovich Jet al. High Mobility Group Box 1 Release from Hepatocytes during Ischemia and Reperfusion Injury Is Mediated by Decreased Histone Deacetylase Activity. J Biol Chem 285:39888-97 (2010). WB; Mouse.Read more (PubMed: 20937823) »
See all 3 publications for this product
Publishing research using ab39408? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"