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Lane 1 : Anti-HDAC5 antibody [HDAC5-35] (ab50001) at 2 µg/ml
Lane 2 : no primary antibody
Lane 1 : 3T3 cell extract (mouse fibroblasts)
Lane 2 : 3T3 cell extract (mouse fibroblasts)
Secondary
Goat Anti-Mouse, Peroxidase conjugate
Predicted band size : 122 kDa
Observed band size : ~122 kDa (why is the actual band size different from the predicted?)
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