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- Secondary antibodies
- Lysates
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Product name
Anti-HDAC5 antibody
See all HDAC5 products (9) ...
Description
Rabbit polyclonal to HDAC5
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Hamster
Immunogen
Synthetic peptide: PREGSTESESTQEDLE, corresponding to amino acids 572-587 of Human HDAC5.
PREGSTESESTQEDLE
Positive control
Jurkat nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - HDAC5 antibody (ab11969)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 120 kDa.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Post-translational
modifications
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
Target information above from: UniProt accessionQ9UQL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- FLJ90614 antibody
see all
Database links
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
Anti-HDAC5 antibody images:
Western blot - HDAC5 antibody (ab11969)
HDAC5 detection by Western blot. The detection of HDAC5 in 293 (lane 1), Jurkat (lane 2) and NIH/3T3 (lane 3) cell extract using the HDAC5 antibody at a 2 µg/ml dilution.
References for Anti-HDAC5 antibody (ab11969)
ab11969 has not yet been referenced specifically in any publications.
Publishing research using ab11969? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"