HDAC5 antibody (ab1439)

Product Name 

HDAC5 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HDAC5

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 20 µg/ml.
IP: Use at a concentration of 20 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 121.9 kDa.
HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.


Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa or COS cell lysate

Cellular localization 

Nuclear and cytoplasmic. Shuttles between the nucleus and the cytoplasm.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs

Relevance 

HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).

See below for HDAC5 antibody images, references, products related to ab1439 and other tools.

HDAC5 antibody images:

References for HDAC5 antibody (ab1439)

  This product has been used in: (two most recent references)

Bierne H et al. Human BAHD1 promotes heterochromatic gene silencing. Proc Natl Acad Sci U S A 106:13826-31 (2009). WB; Human. PubMed: 19666599

Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999). PubMed: 10220385
If you publish research using ab1439 please let us know so that we can cite the reference on this datasheet.

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