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Anti-HDAC5 antibody (ab1439)

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Overview

Product name

Anti-HDAC5 antibody
See all HDAC5 products (9) ...

Description

Rabbit polyclonal to HDAC5

Specificity

The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.

Tested applications

IHC-FoFr, IHC-P, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Positive control

HeLa or COS cell lysate

General notes

Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - HDAC5 antibody (ab1439)Western blot - HDAC5 antibody (ab1439) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-FoFr

     IHC-FoFr: Use at an assa...Read more →

    IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19939859)

  • ShowHide

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 20 µg/ml

  • ShowHide

    IP

     IP: Use a concentration ...Read more →

    IP: Use a concentration of 20 µg/ml

  • ShowHide1 Image

    WB

      See more...Read more →

    WB: Use a concentration of 1 µg/mlPredicted molecular weight: 121.9 kDa.(

    HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.)

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the histone deacetylase family. HD type 2 subfamily.

Domain

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Post-translational
modifications

Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.

Cellular localization

Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.

Target information above from: UniProt accessionQ9UQL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Antigen NY CO 9 antibody
  • Antigen NY-CO-9 antibody
  • FLJ90614 antibody
  • HD 5 antibody
  • HD5 antibody
  • HDAC 5 antibody
  • HDAC5 antibody
  • HDAC5_HUMAN antibody
  • Histone deacetylase 5 antibody
  • KIAA0600 antibody
  • NY CO 9 antibody
see all

Database links

Anti-HDAC5 antibody images:

  Western blot - HDAC5 antibody (ab1439)

Western blot - HDAC5 antibody (ab1439)

All lanes : Anti-HDAC5 antibody (ab1439)

Lane 1 : Cell lysate prepared from HeLa cells
Lane 2 : Cell lysate prepared from HepG2 cells


Predicted band size : 121.9 kDa
Observed band size : 124 kDa (why is the actual band size different from the predicted?)

Protocols

  • Recommended WB protocol with ab1439

    • References for Anti-HDAC5 antibody (ab1439)

    This product has been referenced in:

    • Host Let al. Cocaine self-administration alters the expression of chromatin-remodelling proteins; modulation by histone deacetylase inhibition. J Psychopharmacol 25:222-9 (2011). IHC-FoFr; Rat.Read more (PubMed: 19939859) »
    • Mielcarek Met al. SAHA Decreases HDAC 2 and 4 Levels In Vivo and Improves Molecular Phenotypes in the R6/2 Mouse Model of Huntington's Disease. PLoS One 6:e27746 (2011). WB; Mouse.Read more (PubMed: 22140466) »

    See all 5 publications for this product

    Publishing research using ab1439? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"