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Read our guarantee »Anti-HDAC5 antibody
See all HDAC5 products (9) ...
Rabbit polyclonal to HDAC5
The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.
IHC-FoFr, IHC-P, IP, WBmore details
Reacts with
Mouse, Rat, Human
HeLa or COS cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Western blot - HDAC5 antibody (ab1439)
(enlarge)
Our Abpromise guarantee covers the use of ab1439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19939859)
IHC-P: Use a concentration of 20 µg/ml
IP: Use a concentration of 20 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 121.9 kDa.(HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.)
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Ubiquitous.
Belongs to the histone deacetylase family. HD type 2 subfamily.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
Target information above from: UniProt accessionQ9UQL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - HDAC5 antibody (ab1439)

All lanes : Anti-HDAC5 antibody (ab1439)
Lane 1 : Cell lysate prepared from HeLa cells
Lane 2 : Cell lysate prepared from HepG2 cells
Predicted band size : 121.9 kDa
Observed band size : 124 kDa (why is the actual band size different from the predicted?)
This product has been referenced in:
See all 5 publications for this product
Publishing research using ab1439? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-HDAC5 antibody (ab1439)
Lane 1 : Cell lysate prepared from HeLa cells
Lane 2 : Cell lysate prepared from HepG2 cells
Predicted band size : 121.9 kDa
Observed band size : 124 kDa (why is the actual band size different from the predicted?)
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