Overview

  • Product nameAnti-HDAC5 antibodySee all HDAC5 primary antibodies ...
  • Description
    Rabbit polyclonal to HDAC5
  • SpecificityThe antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.
  • Tested applicationsIHC-FoFr, IHC-P, IP, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HDAC5.

  • EpitopeThe immunogen was a synthetic peptide surrounding amino acid 575 of human HDAC-5
  • Positive control
    • HeLa or COS cell lysate
  • General notesSome tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).

Properties

Applications

Our Abpromise guarantee covers the use of ab1439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 19939859
IHC-P Use a concentration of 20 µg/ml.
IP Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121.9 kDa.
HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Target information above from: UniProt accession Q9UQL6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 9 antibody
    • Antigen NY-CO-9 antibody
    • FLJ90614 antibody
    • HD 5 antibody
    • HD5 antibody
    • HDAC 5 antibody
    • HDAC5 antibody
    • HDAC5_HUMAN antibody
    • Histone deacetylase 5 antibody
    • KIAA0600 antibody
    • NY CO 9 antibody
    see all

Anti-HDAC5 antibody images

  • All lanes : Anti-HDAC5 antibody (ab1439) at 1/1000 dilution

    Lane 1 : Rat hippocampus cell lysate
    Lane 2 : Rat hippocampus cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Polyclonal anti-rabbit IgG HRP conjugate at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 121.9 kDa


    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Seungyeon Ko

    See Abreview

  • All lanes : Anti-HDAC5 antibody (ab1439)

    Lane 1 : Cell lysate prepared from HeLa cells
    Lane 2 : Cell lysate prepared from HepG2 cells


    Predicted band size : 121.9 kDa
    Observed band size : 124 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of  HDAC5 in HeLa  (Lane1) and HepG2 (Lane2) cell lysates. A band of approximately 124 KDa can be detected.

References for Anti-HDAC5 antibody (ab1439)

This product has been referenced in:
  • Host L  et al. Cocaine self-administration alters the expression of chromatin-remodelling proteins; modulation by histone deacetylase inhibition. J Psychopharmacol 25:222-9 (2011). IHC-FoFr ; Rat . Read more (PubMed: 19939859) »
  • Mielcarek M  et al. SAHA Decreases HDAC 2 and 4 Levels In Vivo and Improves Molecular Phenotypes in the R6/2 Mouse Model of Huntington's Disease. PLoS One 6:e27746 (2011). WB ; Mouse . Read more (PubMed: 22140466) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (9% gel)
Sample Rat Cell lysate - whole cell (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (9% gel)
Sample Mouse Tissue lysate - whole (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 30 2013

Application Western blot
Sample Rat Cell lysate - whole cell (Hippocampus)
Loading amount 15 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10% SDS gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Seungyeon Ko

Verified customer

Submitted May 14 2013

Application Western blot
Sample Rabbit Cell lysate - whole cell (hippocampus)
Loading amount 25 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (10%SDS)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Sung-Eun WANG

Verified customer

Submitted Apr 17 2013

Thank you for contacting us.

SInce this is a polyclonal antibody and not a monoclonal, it will bind to many places on the protein and not to one particular site. Polyclonal antibodies are generally not epitope mapped, and not all of are monocl...

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Thank you for inquiry.

I can confirm that tghe immunogen for ab1439 was a synthetic peptide surrounding amino acid 575 of human HDAC-5.

I also included this information on the datasheet for your information.

I hope this in...

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Thank you for your  reply. I am sending a free of charge vial of ab13939 on the order ***. This product is in stock and will arrive on Monday. Please let me know if you have any questions or if there is anything else that we can do for you.

Thanks for letting me know and keeping me updated. I am sorry to hear that this replacement antibody did not work either. I would be happy to send a different antibody to try or issue a credit or refund. I am running into a meeting but I can gi...

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Thank you for your call today and for letting us know about the problem with ab1439. As we discussed, I am sending a free of charge vial of ab50001 on the order ***, which should arrive shortly. Please keep me updated of the results with this r...

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Thank you for your enquiry. We currently only have two HDAC5 antibodies, ab1439 and ab11969. I'm not sure if a phospho-specific antibody is available, but to see if another company has one, you can click on the link to "The World's Antibody Gateway". T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"