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Read our guarantee »Anti-HDAC5 (phospho S259) antibody
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Rabbit polyclonal to HDAC5 (phospho S259)
ab53693 detects endogenous levels of HDAC5 only when phosphorylated at serine 259.
IHC-FoFr, IP, WB, ELISAmore details
Reacts with
Rat, Human
Predicted to work with
Mouse
Synthetic phosphopeptide derived from human HDAC5 around the phosphorylation site of serine 259 (T-A-SP-E-P).
HepG2 cell extracts.
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab53693 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Polyclonal
IgG
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Western blot - HDAC5 (phospho S259) antibody (ab53693)
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Our Abpromise guarantee covers the use of ab53693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19939859)
IP: Use at an assay dependent concentration.
WB: 1/500 - 1/1000.Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
ELISA: 1/5000
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Ubiquitous.
Belongs to the histone deacetylase family. HD type 2 subfamily.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
Target information above from: UniProt accessionQ9UQL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - HDAC5 (phospho S259) antibody (ab53693)

All lanes : Anti-HDAC5 (phospho S259) antibody (ab53693) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with the immunising peptide
Predicted band size : 122 kDa
Observed band size : 122 kDa
This product has been referenced in:
See all 2 publications for this product
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