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Western blot - HDAC5 (phospho S259) antibody (ab53693)
Western blot - HDAC5 (phospho S259) antibody (ab53693)
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Product Name 

HDAC5 (phospho S259) antibody

 

See all HDAC5 antibodies (10)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HDAC5 (phospho S259)

Immunogen 

Synthetic phosphopeptide derived from human HDAC5 around the phosphorylation site of serine 259 (T-A-SP-E-P).

Reacts with 
(species key)

Hu, Ms

Specificity 

ab53693 detects endogenous levels of HDAC5 only when phosphorylated at serine 259.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell extracts.

Cellular localization 

Nuclear and cytoplasmic. Shuttles between the nucleus and the cytoplasm.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs

Relevance 

HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.

 

Alternative names HDAC5 (phospho S259) antibody (ab53693)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10014    (Human)
15184    (Mouse)

Omim

    

605315    (Human)

SwissProt

    

Q9UQL6    (Human)
Q9Z2V6    (Mouse)

Unigene

    

438782    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab53693 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC5 (phospho S259) antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for HDAC5 (phospho S259) antibody images, references, products related to ab53693 and other tools.

 

HDAC5 (phospho S259) antibody images:

Product image  Western blot - HDAC5 (phospho S259) antibody (ab53693)

Western blot - HDAC5 (phospho S259) antibody (ab53693)

All lanes : HDAC5 (phospho S259) antibody (ab53693) at 1/500 dilution

Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with the immunising peptide


Predicted band size : 122 kDa
Observed band size : 122 kDa

 
 

Search PubMed (MEDLINE) for references to HDAC5

 

HDAC5 (phospho S259) antibody - more information

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HDAC5 (phospho S259) antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for HDAC5 (phospho S259) antibody

ab14659

  

HepG2 (Human hepatocellular liver carcinoma cell line) Cytoplasmic Lysate

ab14660

  

HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

 

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Other HDAC5 Antibodies

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HDAC5 (phospho S259) antibody - other tools:

Contact Abcam with a Technical Enquiry about HDAC5 (phospho S259) antibody

 

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