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Overview
Product name
Anti-HDAC5 (phospho S259) antibody
See all HDAC5 products (9) ...
Description
Rabbit polyclonal to HDAC5 (phospho S259)
Specificity
ab53693 detects endogenous levels of HDAC5 only when phosphorylated at serine 259.
Tested applications
IHC-FoFr, IP, WB, ELISAmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from human HDAC5 around the phosphorylation site of serine 259 (T-A-SP-E-P).
Positive control
HepG2 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53693 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - HDAC5 (phospho S259) antibody (ab53693)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19939859)
IP: Use at an assay dependent concentration.
WB: 1/500 - 1/1000.Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Post-translational
modifications
Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
Target information above from: UniProt accessionQ9UQL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- FLJ90614 antibody
see all
Database links
- Entrez Gene: 10014 Human
- Entrez Gene: 15184 Mouse
- Entrez Gene: 84580 Rat
- Omim: 605315 Human
- SwissProt: Q9UQL6 Human
- SwissProt: Q9Z2V6 Mouse
- Unigene: 438782 Human
- Unigene: 22665 Mouse
Anti-HDAC5 (phospho S259) antibody images:
Western blot - HDAC5 (phospho S259) antibody (ab53693)
All lanes : Anti-HDAC5 (phospho S259) antibody (ab53693) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with the immunising peptide
Predicted band size : 122 kDa
Observed band size : 122 kDa
References for Anti-HDAC5 (phospho S259) antibody (ab53693)
This product has been referenced in:
- Host Let al. Cocaine self-administration alters the expression of chromatin-remodelling proteins; modulation by histone deacetylase inhibition. J Psychopharmacol 25:222-9 (2011). IHC-FoFr; Rat.Read more (PubMed: 19939859) »
- Zhao JXet al. AMP-activated protein kinase regulates beta-catenin transcription via histone deacetylase 5. J Biol Chem 286:16426-34 (2011). WB; Mouse.Read more (PubMed: 21454484) »
See all 2 publications for this product
Publishing research using ab53693? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"