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Overview
Product name
Anti-HDAC6 antibody - ChIP Grade
See all HDAC6 products (13) ...
Description
Rabbit polyclonal to HDAC6 - ChIP Grade
Tested applications
IP, ChIP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: MTSTGQDSTT TRQRRS, corresponding to N terminal amino acids 1-16 of Human HDAC6
MTSTGQDSTT TRQRRS
Positive control
NIH-3T3 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - HDAC6 antibody - ChIP Grade (ab47181)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
ChIP
Application notes
ChIP: Use at an assay dependent dilution. Refer to Imbriano (2005, Fig 7) for details.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 134 kDa. (Predicted molecular weight: 131 kDa.)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
Post-translational
modifications
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Target information above from: UniProt accessionQ9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16239 antibody
- HD 6 antibody
- HD6 antibody
see all
Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
Anti-HDAC6 antibody - ChIP Grade images:
Western blot - HDAC6 antibody - ChIP Grade (ab47181)
Anti-HDAC6 antibody - ChIP Grade (ab47181) at 2 µg/ml + NIH-3T3 cell lysate
Predicted band size : 131 kDa
Observed band size : 134 kDa (why is the actual band size different from the predicted?)
References for Anti-HDAC6 antibody - ChIP Grade (ab47181)
This product has been referenced in:
- Imbriano Cet al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-51 (2005).Read more (PubMed: 15831478) »
See 1 publication for this product
Publishing research using ab47181? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"