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Western blot - HDAC6 antibody - ChIP Grade (ab47181)
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Product Name
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HDAC6 antibody - ChIP Grade
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See all HDAC6 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HDAC6 - ChIP Grade
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Immunogen
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Synthetic peptide: MTSTGQDSTT TRQRRS, corresponding to N terminal amino acids 1-16 of Human HDAC6
BLAST 'MTSTGQDSTTTRQRRS' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ChIP, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution. Refer to Imbriano (2005, Fig 7) for details.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 134 kDa. (Predicted molecular weight: 131 kDa.)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH-3T3 cell lysate.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
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Relevance
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HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC6 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HDAC6 antibody - ChIP Grade images, references, products related to ab47181 and other tools.
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HDAC6 antibody - ChIP Grade images:
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References for HDAC6 antibody - ChIP Grade (ab47181)
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This product has been used in:
Imbriano C et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-51 (2005). PubMed: 15831478
If you publish research using ab47181 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HDAC6
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HDAC6 antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding HDAC6 antibody - ChIP Grade |
Customer FAQs regarding HDAC6 antibody - ChIP Grade |
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HDAC6 antibody - ChIP Grade - related products:
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HDAC6 antibody - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about HDAC6 antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - HDAC6 antibody - ChIP Grade (ab47181)