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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab12173? Please let us know so that we can cite the reference in this datasheet
ab12173 has been referenced in 2 publications.
- Ouyang H et al. Protein Aggregates Are Recruited to Aggresome by Histone Deacetylase 6 via Unanchored Ubiquitin C Termini. J Biol Chem 287:2317-27 (2012). PubMed: 22069321
- Alamdari N et al. Sepsis and glucocorticoids upregulate p300 and downregulate HDAC6 expression and activity in skeletal muscle. Am J Physiol Regul Integr Comp Physiol 299:R509-20 (2010). WB. PubMed: 20538901
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HDAC6 antibody (ab12173)
All lanes : Anti-HDAC6 antibody (ab12173) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from Human HepG2 cell line.
Lane 2 : Whole cell lysate prepared from Human HepG2 cell line.
Lane 3 : Whole cell lysate prepared from Human HepG2 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Cheng Song
Immunocytochemistry/ Immunofluorescence - HDAC6 antibody (ab12173)
ab12173 staining mouse NIH3T3 cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C . The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C . A Alexa Fluor® 488 goat polyclonal to rabbit IgG was used as the secondary, diluted 1/200.
This image is courtesy of an anonymous Abreview
HDAC6 antibody for WB in Human (12173)
HDAC6 antibody for ICC/IF in Mouse (12173)
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