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Western blot - HDAC6 antibody (ab12173)
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Immunocytochemistry/ Immunofluorescence - HDAC6 antibody (ab12173)
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Product Name
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HDAC6 antibody
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See all HDAC6 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HDAC6
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Immunogen
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Synthetic peptide: KNIAHQNKFGEDMPH conjugated to KLH, corresponding to amino acids 1199-1213 of Human HDAC6.
BLAST 'KNIAHQNKFGEDMPH' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a dilution of 1/50 for 1 hr.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 134 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cells expressing recombinant mouse HDAC6.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
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Relevance
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HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Phosphate buffered saline
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Form
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Liquid
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Concentration
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Batch dependent within range: 5.90 - 5.90 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HDAC6 antibody images, references, products related to ab12173 and other tools.
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HDAC6 antibody images:
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 Western blot - HDAC6 antibody (ab12173)
All lanes : HDAC6 antibody (ab12173) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from Human HepG2 cell line.
Lane 2 : Whole cell lysate prepared from Human HepG2 cell line.
Lane 3 : Whole cell lysate prepared from Human HepG2 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute This image is courtesy of an Abreview submitted by Cheng Song
See Abreview
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Immunocytochemistry/ Immunofluorescence - HDAC6 antibody (ab12173)
ab12173 staining mouse NIH3T3 cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C . The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C . A Alexa Fluor® 488 goat polyclonal to rabbit IgG was used as the secondary, diluted 1/200. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to HDAC6
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HDAC6 antibody - more information
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Customer reviews (feedback) regarding HDAC6 antibody |
Customer FAQs regarding HDAC6 antibody |
Protocols for HDAC6 antibody |
Price and availability of products related to HDAC6 antibody |
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HDAC6 antibody - related products:
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HDAC6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HDAC6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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