Anti-HDAC6 antibody (ab1440)
Key features and details
- Rabbit polyclonal to HDAC6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HDAC6 antibody
See all HDAC6 primary antibodies -
Description
Rabbit polyclonal to HDAC6 -
Host species
Rabbit -
Specificity
The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Mouse) - N terminal.
-
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3606 HDAC6 Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa). Block with 5% milk in TBST and incubate the antibody in blocking buffer.
|
Notes |
---|
WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa). Block with 5% milk in TBST and incubate the antibody in blocking buffer. |
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
-
Database links
- Entrez Gene: 10013 Human
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- Unigene: 6764 Human
-
Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (30)
ab1440 has been referenced in 30 publications.
- Kang MJ et al. Metformin induces muscle atrophy by transcriptional regulation of myostatin via HDAC6 and FoxO3a. J Cachexia Sarcopenia Muscle 13:605-620 (2022). PubMed: 34725961
- Megino-Luque C et al. ARID1A-deficient cells require HDAC6 for progression of endometrial carcinoma. Mol Oncol 16:2235-2259 (2022). PubMed: 35167193
- Litke C et al. Organic anion transporter 1 is an HDAC4-regulated mediator of nociceptive hypersensitivity in mice. Nat Commun 13:875 (2022). PubMed: 35169129
- Yano M et al. Up-regulation of HDAC6 Results in Poor Prognosis and Chemoresistance in Patients With Advanced Ovarian High-grade Serous Carcinoma. Anticancer Res 41:1647-1654 (2021). PubMed: 33788761
- Guo SD et al. HDAC6 promotes sepsis development by impairing PHB1-mediated mitochondrial respiratory chain function. Aging (Albany NY) 12:5411-5422 (2020). PubMed: 32221047