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Overview
Product name
Anti-HDAC6 antibody
See all HDAC6 products (13) ...
Description
Rabbit polyclonal to HDAC6
Specificity
The antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Mouse) - N terminal.
Positive control
mouse small intestine lysate, jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - HDAC6 antibody (ab1440)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 20 µg/ml
WB: Use a concentration of 0.5 - 4 µg/ml.Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). (Block with 5% milk in TBST and incubate the antibody in blocking buffer.)
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
Post-translational
modifications
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Target information above from: UniProt accessionQ9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16239 antibody
- HD 6 antibody
- HD6 antibody
see all
Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Entrez Gene: 84581 Rat
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
Anti-HDAC6 antibody images:
Western blot - HDAC6 antibody (ab1440)
Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate
Predicted band size : 131 kDa
Observed band size : 131 kDa
References for Anti-HDAC6 antibody (ab1440)
This product has been referenced in:
- Wei LHet al. Histone deacetylase 6 regulates estrogen receptor alpha in uterine leiomyoma. Reprod Sci 18:755-62 (2011).Read more (PubMed: 21421902) »
- Chen Set al. HDAC6 regulates mitochondrial transport in hippocampal neurons. PLoS One 5:e10848 (2010).Read more (PubMed: 20520769) »
See all 7 publications for this product
Publishing research using ab1440? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"