Overview
- Product nameAnti-HDAC6 antibodySee all HDAC6 primary antibodies ...
- DescriptionRabbit polyclonal to HDAC6
- SpecificityThe antibody detects ~134 kDa histone deacetylase 6. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
- Tested applicationsIHC-P, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide (Mouse) - N terminal.
- Positive controlmouse small intestine lysate, jurkat cell lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
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Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab1440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use a concentration of 20 µg/ml. |
| WB | WB: Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 131 kDa).Can be blocked with HDAC6 peptide (ab41708). Block with 5% milk in TBST and incubate the antibody in blocking buffer. |
Target
- FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
- Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. - Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. - Cellular localizationNucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
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Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 10013 Human
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Entrez Gene: 84581 Rat
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
- Unigene: 13453 Rat
see all
Target information above from: UniProt accession
Q9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- FLJ16239 antibodyHD 6 antibodyHD6 antibody
- HDAC 6 antibodyHDAC6 antibodyHDAC6_HUMAN antibodyHistone deacetylase 6 (HD6) antibodyHistone deacetylase 6 (HD6). antibodyHistone deacetylase 6 antibodyJM 21 antibodyJM21 antibodyKIAA0901 antibodyOTTHUMP00000032398 antibodyOTTHUMP00000197663 antibody
see all
Anti-HDAC6 antibody images
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Western blot - HDAC6 antibody (ab1440)Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate
Predicted band size : 131 kDa
Observed band size : 131 kDa
References for Anti-HDAC6 antibody (ab1440)
This product has been referenced in:
- Wei LH et al. Histone deacetylase 6 regulates estrogen receptor alpha in uterine leiomyoma. Reprod Sci 18:755-62 (2011). Read more (PubMed: 21421902) »
- Chen S et al. HDAC6 regulates mitochondrial transport in hippocampal neurons. PLoS One 5:e10848 (2010). Read more (PubMed: 20520769) »
