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Overview
Product name
Anti-HDAC6 antibody
See all HDAC6 products (13) ...
Description
Rabbit polyclonal to HDAC6
Specificity
ab53099 detects endogenous levels of total HDAC6 protein
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from human HDAC6.
Positive control
WB: HeLa cell extract.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53099 as affinity purified from rabbit antiserum by peptide affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - HDAC6 antibody (ab53099)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
Post-translational
modifications
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Target information above from: UniProt accessionQ9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16239 antibody
- HD 6 antibody
- HD6 antibody
see all
Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
Anti-HDAC6 antibody images:
Western blot - HDAC6 antibody (ab53099)
All lanes : Anti-HDAC6 antibody (ab53099) at 1/300 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with peptide
Predicted band size : 131 kDa
Observed band size : 131 kDa
References for Anti-HDAC6 antibody (ab53099)
ab53099 has not yet been referenced specifically in any publications.
Publishing research using ab53099? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"