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- Secondary antibodies
- Lysates
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Product name
Anti-HDAC6 antibody
See all HDAC6 products (13) ...
Description
Rabbit polyclonal to HDAC6
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region within amino acids between 850-900 of Human HDAC6 (NP_006035.2).
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab99336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 131 kDa.
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
Post-translational
modifications
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Target information above from: UniProt accessionQ9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16239 antibody
- HD 6 antibody
- HD6 antibody
see all
Database links
- Entrez Gene: 10013 Human
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- Unigene: 6764 Human
Anti-HDAC6 antibody images:
Western blot - HDAC6 antibody (ab99336)
All lanes : Anti-HDAC6 antibody (ab99336) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 131 kDa
Exposure time : 30 seconds
Immunoprecipitation - HDAC6 antibody (ab99336)
ab99336 at 1µg/ml detecting HDAC6 in HeLa whole cell lysate by WB after IP.
Lane 1: IP ab99336 at 3µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of HDAC6
Lane 3: control IgG IP.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-HDAC6 antibody (ab99336)
ab99336 has not yet been referenced specifically in any publications.
Publishing research using ab99336? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"