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Product name
Anti-HDAC6 (phospho S22) antibody
See all HDAC6 products (13) ...
Description
Rabbit polyclonal to HDAC6 (phospho S22)
Specificity
ab61058 detects endogenous levels of HDAC6 only when phosphorylated at serine 22. (Human: Ser22; Mouse: Ser21).
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of serine 22 (PQSpPP).
Positive control
Human testis tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61058 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC6 (phospho S22) antibody (ab61058)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000.ELISA: 1/5000.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
Post-translational
modifications
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules.
Target information above from: UniProt accessionQ9UBN7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16239 antibody
- HD 6 antibody
- HD6 antibody
see all
Database links
- Entrez Gene: 10013 Human
- Entrez Gene: 15185 Mouse
- Omim: 300272 Human
- SwissProt: Q9UBN7 Human
- SwissProt: Q9Z2V5 Mouse
- Unigene: 6764 Human
- Unigene: 29854 Mouse
Anti-HDAC6 (phospho S22) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC6 (phospho S22) antibody (ab61058)
Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (phospho S22) antibody (ab61058) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of phosphopeptide.
References for Anti-HDAC6 (phospho S22) antibody (ab61058)
This product has been referenced in:
- Chen S et al. HDAC6 regulates mitochondrial transport in hippocampal neurons. PLoS One 5:e10848 (2010).Read more (PubMed: 20520769) »
See 1 publication for this product
Publishing research using ab61058? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"