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Read our guarantee »Overview
Product name
Anti-HDAC8 antibody [HDAC8-48]
See all HDAC8 products (7) ...
Description
Mouse monoclonal [HDAC8-48] to HDAC8
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Human).
Positive control
HeLa cell nuclear extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
HDAC8-48
Isotype
IgG1
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 4 µg/ml. Detects a band of approximately 43 kDa.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
Tissue specificity
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
Cellular localization
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
Target information above from: UniProt accessionQ9BY41
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HD 8 antibody
- HD8 antibody
- HDAC 8 antibody
see all
Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- Unigene: 112272 Human
- Unigene: 310536 Human
- Unigene: 328128 Mouse
References for Anti-HDAC8 antibody [HDAC8-48] (ab12176)
This product has been referenced in:
- Colussi C et al. HDAC2 blockade by nitric oxide and histone deacetylase inhibitors reveals a common target in Duchenne muscular dystrophy treatment. Proc Natl Acad Sci U S A 105:19183-7 (2008). WB; Mouse.Read more (PubMed: 19047631) »
See 1 publication for this product
Publishing research using ab12176? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"