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Anti-HDAC8 antibody (ab23658)

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Overview

Product name

Anti-HDAC8 antibody
See all HDAC8 products (5) ...

Description

Rabbit polyclonal to HDAC8

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human HDAC8.

(Peptide available as ab27458.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - HDAC8 antibody (ab23658)Western blot - HDAC8 antibody (ab23658) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab23658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.

Tissue specificity

Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

Cellular localization

Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.

Target information above from: UniProt accessionQ9BY41 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HD 8 antibody
  • HD8 antibody
  • HDAC 8 antibody
  • Hdac8 antibody
  • HDAC8_HUMAN antibody
  • HDACL 1 antibody
  • HDACL1 antibody
  • HDACL1 antibody
  • Histone deacetylase 8 antibody
  • Histone deacetylase 8 antibody
  • Histone deacetylase like 1 antibody
  • RPD 3 antibody
  • RPD3 antibody
  • RPD3 antibody
see all

Anti-HDAC8 antibody images:

  Western blot - HDAC8 antibody (ab23658)

Western blot - HDAC8 antibody (ab23658)

All lanes : Anti-HDAC8 antibody (ab23658) at 1 µg/ml

Lane 1 : HeLa Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 62 kDa (possible non-specific binding),74 kDa (possible non-specific binding).

See Abreview

References for Anti-HDAC8 antibody (ab23658)

ab23658 has not yet been referenced specifically in any publications.

Publishing research using ab23658? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"