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Western blot - HDAC8 antibody (ab23658)
Western blot - HDAC8 antibody (ab23658)
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Product Name 

HDAC8 antibody

 

See all HDAC8 antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to HDAC8

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human HDAC8.



(Peptide available as ab27458.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nucleus. Excluded from the nucleoli.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

 

Alternative names HDAC8 antibody (ab23658)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

55869    (Human)
70315    (Mouse)

Omim

    

300269    (Human)

SwissProt

    

Q9BY41    (Human)
Q8VH37    (Mouse)

Unigene

    

112272    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC8 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for HDAC8 antibody images, references, products related to ab23658 and other tools.

 

HDAC8 antibody images:

Product image  Western blot - HDAC8 antibody (ab23658)

Western blot - HDAC8 antibody (ab23658)

All lanes : HDAC8 antibody (ab23658) at 1 µg/ml

Lane 1 : HeLa Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 62 kDa (possible non-specific binding),74 kDa (possible non-specific binding).

 
 

Search PubMed (MEDLINE) for references to HDAC8

 

HDAC8 antibody - more information

Customer reviews (feedback) regarding HDAC8 antibody

Customer FAQs regarding HDAC8 antibody

Protocols for HDAC8 antibody

Price and availability of products related to HDAC8 antibody

 

HDAC8 antibody - related products:

Products for Western blot

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Lysates

 

Immunizing Peptide for HDAC8 antibody

ab27458

  

HDAC8 peptide

 

Compatible Secondaries for HDAC8 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal Secondary Antibody to Rabbit IgG - H&L

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other HDAC8 Antibodies

See all HDAC8 antibodies (5)...

 

HDAC8 antibody - other tools:

Contact Abcam with a Technical Enquiry about HDAC8 antibody

 

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