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Product name
Anti-HDAC8 antibody
See all HDAC8 products (7) ...
Description
Rabbit polyclonal to HDAC8
Specificity
ab39664 detects endogenous levels of total HDAC8 protein.
Tested applications
ICC/IF, IP, WB, IHC-P, ELISA, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) non-phosphopeptide derived from HDAC8, around the phosphorylation site of Serine 39.
Positive control
Lung carcinoma and NIH/3T3 cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. PubMed: 21803775
IP: Use at an assay dependent concentration. PubMed: 21803775
WB: 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-FoFr: Use at an assay dependent dilution. PubMed: 21526203
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
Tissue specificity
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
Cellular localization
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
Target information above from: UniProt accessionQ9BY41
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HD 8 antibody
- HD8 antibody
- HDAC 8 antibody
see all
Database links
- Entrez Gene: 55869 Human
- Entrez Gene: 70315 Mouse
- Entrez Gene: 363481 Rat
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- SwissProt: Q8VH37 Mouse
- SwissProt: B1WC68 Rat
- Unigene: 112272 Human
see all
Anti-HDAC8 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - HDAC8 antibody (ab39664)
Immunohistochemical analysis of paraffin embedded lung carcinoma tissue, using ab39664. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC8 antibody (ab39664)
ab39664 staining HDAC8 (red arrows) in human myometrial cells by Immunocytochemistry/ Immunofluorescence. Counterstained with DAPI (white arrows).
Image from Karolczak-Bayatti M et al, J Biol Chem. 2011 Sep 30;286(39):34346-55. Epub 2011 Jul 29, Fig 1. DOI 10.1074/jbc.M111.278549
Western blot - HDAC8 antibody (ab39664)
All lanes : Anti-HDAC8 antibody (ab39664)
Lane 1 : NIH/3T3 Cells
Lane 2 : NIH/3T3 Cells with synthesized peptide
Predicted band size : 42 kDa
Observed band size : 42 kDa
Immunoprecipitation - Anti-HDAC8 antibody (ab39664)
HDAC8 was immunoprecipitated using 0.5mg NIH3T3 whole cell lysate, 5µg of Rabbit polyclonal to HDAC8 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab39664.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 48kDa: HDAC8.
References for Anti-HDAC8 antibody (ab39664)
This product has been referenced in:
- Funato H et al. Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus. PLoS One 6:e18950 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21526203) »
- Karolczak-Bayatti M et al. Acetylation of heat shock protein 20 (hsp20) regulates human myometrial activity. J Biol Chem 286:34346-55 (2011). WB, ICC/IF, IP; Human.Read more (PubMed: 21803775) »
See all 2 publications for this product
Publishing research using ab39664? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"