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Product name
Anti-HDAC9 antibody - ChIP Grade
See all HDAC9 products (7) ...
Description
Rabbit polyclonal to HDAC9 - ChIP Grade
Specificity
This antibody recognises Histone deacetylase 9, isoform CRA b/isoform 3, also known as HDRP.
Tested applications
WB, IHC-P, ChIP, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Dog
Immunogen
A region within synthetic peptide: QVGAVKVKEE PVDSDEDAQI QEMESGEQAA FMQQVIGKDL APGFVIKVII, corresponding to C terminal amino acids 541-590 of Human HDAC9
QVGAVKVKEEPVDSDEDAQIQEMESGEQAAFMQQVIGKDL APGFVIKVII
Positive control
A172 cell lysate or human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 66 kDa).Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P: Use a concentration of 4 - 8 µg/ml.
ChIP: Use at an assay dependent dilution. PubMed: 20596014
ELISA: Use at an assay dependent dilution.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
Tissue specificity
Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
Involvement in disease
Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Post-translational
modifications
Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Sumoylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UKV0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp779K1053 antibody
- HD 7 antibody
- HD 7B antibody
see all
Database links
- Entrez Gene: 9734 Human
- Entrez Gene: 79221 Mouse
- Omim: 606543 Human
- SwissProt: Q9UKV0 Human
- SwissProt: Q99N13 Mouse
- Unigene: 196054 Human
- Unigene: 310551 Mouse
- Unigene: 483009 Mouse
Anti-HDAC9 antibody - ChIP Grade images:
Western blot - HDAC9 antibody (ab59718)
Anti-HDAC9 antibody - ChIP Grade (ab59718) at 1.25 µg/ml + A172 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG used at 1/50000
Predicted band size : 66 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - HDAC9 antibody (ab59718)
Ab59718 (4ug/ml) staining human HDAC9 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
Epithelial cells of the renal tubule were positively labelled (indicated with arrows).
References for Anti-HDAC9 antibody - ChIP Grade (ab59718)
This product has been referenced in:
- Milde T et al. HDAC5 and HDAC9 in medulloblastoma: novel markers for risk stratification and role in tumor cell growth. Clin Cancer Res 16:3240-52 (2010). IHC-P; Human.Read more (PubMed: 20413433) »
- Hang CT et al. Chromatin regulation by Brg1 underlies heart muscle development and disease. Nature 466:62-7 (2010). WB, ChIP, IP; Mouse.Read more (PubMed: 20596014) »
See all 2 publications for this product
Publishing research using ab59718? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"