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Product name
Anti-HDAC9 antibody
See all HDAC9 products (7) ...
Description
Rabbit polyclonal to HDAC9
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Chicken, Dog, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HDAC9.
Positive control
This antibody gave a positive signal in Human testis tissue lysate and MCF7 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - HDAC9 antibody (ab86140)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
Tissue specificity
Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
Involvement in disease
Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Post-translational
modifications
Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Sumoylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UKV0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp779K1053 antibody
- HD 7 antibody
- HD 7B antibody
see all
Database links
- Entrez Gene: 9734 Human
- Omim: 606543 Human
- SwissProt: Q9UKV0 Human
- Unigene: 196054 Human
Anti-HDAC9 antibody images:
Western blot - HDAC9 antibody (ab86140)
All lanes : Anti-HDAC9 antibody (ab86140) at 1 µg/ml
Lane 1 :
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 150 kDa
Observed band size : 150 kDa
Additional bands at : 270 kDa,72 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-HDAC9 antibody (ab86140)
ab86140 has not yet been referenced specifically in any publications.
Publishing research using ab86140? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"