Anti-HDGF antibody [26C4a] (ab58644)
Key features and details
- Mouse monoclonal [26C4a] to HDGF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-HDGF antibody [26C4a]
See all HDGF primary antibodies -
Description
Mouse monoclonal [26C4a] to HDGF -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment of human HDGF, from an internal sequence.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab58644 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
26C4a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the HDGF family.
Contains 1 PWWP domain. -
Domain
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured. -
Post-translational
modificationsSumoylated by SUMO1. Sumoylation prevents binding to chromatin. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Alternative names
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
see all
Images
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Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein
at 25 µg
Secondary
Sheep Anti Mouse IgG at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The immunogen shown in the WB is tagged.
Datasheets and documents
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Datasheet download
References (3)
ab58644 has been referenced in 3 publications.
- Hong YG et al. MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF. Biomed J 43:163-173 (2020). PubMed: 32418767
- Leich E et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011). IHC-P ; Human . PubMed: 21960592
- Maschietto M et al. Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset. Cell Death Dis 2:e224 (2011). PubMed: 22048167