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Product name
Anti-HE4 antibody
See all HE4 products (5) ...
Description
Rabbit polyclonal to HE4
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.
Positive control
Normal human epididymis or human ovarian tumour.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HE4 antibody (ab24480)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 13 kDa).
IHC-P: 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Tissue specificity
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
Sequence similarities
Contains 2 WAP domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ14508
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 5 isoforms produced by alternative splicing.
Alternative names
- dJ461P17.6 antibody
- EDDM4 antibody
- epididymal protein 4 antibody
see all
Database links
- Entrez Gene: 10406 Human
- SwissProt: Q14508 Human
- Unigene: 2719 Human
Anti-HE4 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HE4 antibody (ab24480)
ab24480 (4µg/ml) staining HE4 in human lung using an automated system (DAKO Autostainer Plus).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-HE4 antibody (ab24480)
This product has been referenced in:
- Tokuishi K et al. Splice variant HE4-V3 expression is associated with favorable prognosis in pulmonary adenocarcinoma. Tumour Biol 33:103-9 (2012). WB, IHC-P; Human.Read more (PubMed: 22015929) »
- Yamashita S et al. Prognostic significance of HE4 expression in pulmonary adenocarcinoma. Tumour Biol 32:265-71 (2011). ICC/IF; Human.Read more (PubMed: 20953751) »
See all 2 publications for this product
Publishing research using ab24480? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"