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Overview

  • Product nameHEC1 protein (Tagged-His Tag)See all HEC1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 350-642 (293 amino acids) of Human HEC1 with N terminal His tag; MWt 37 kDa (UniProt O14777).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 68 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEA EQQKLWNEE LKYARGKEAIETQLAEYHKLARKLKLIPK GAENSKGYDFEIKFNPEAGA NCLVKYRAQVYVPLKELL NETEEEINKALNKKMGLEDTLEQLNAMITES KRSVRTL KEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLE ST VNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNN LQRLLEMVATHVG SVEKHLEEQIAKVDREYEECMSEDL SENIKEIRDKYEKKATLIKSSEE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91996 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        HECHighly expressed in cancerHighly expressed in cancer protein
        Highly expressed in cancer rich in leucine heptad repeatsHsHec1hsNDC80Kinetochore associated 2Kinetochore associated protein 2Kinetochore protein Hec1Kinetochore protein NDC80 homologKinetochore-associated protein 2KNTC2NDC80NDC80 homolog kinetochore complex componentNDC80 kinetochore complex component homologNDC80_HUMANRetinoblastoma associated protein HECRetinoblastoma-associated protein HECTID3
      see all
  • FunctionActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • Sequence similaritiesBelongs to the NDC80/HEC1 family.
  • Developmental stageExpression peaks in mitosis.
  • Post-translational
    modifications    Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.

    • Target information above from: UniProt accession O14777 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    HEC1 protein (Tagged-His Tag) images

    • SDS-PAGE - HEC1 protein (Tagged-His Tag) (ab91996)
      SDS-PAGE - HEC1 protein (Tagged-His Tag) (ab91996)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for HEC1 protein (Tagged-His Tag) (ab91996)

    ab91996 has not yet been referenced specifically in any publications.

    Publishing research using ab91996 ? Please let us know so that we can cite the reference in this datasheet

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