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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab85832? Please let us know so that we can cite the reference in this datasheet
ab85832 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - HERC2 antibody (ab85832)
All lanes : Anti-HERC2 antibody (ab85832) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
developed using the ECL technique
Predicted band size : 527 kDa
Observed band size : 527 kDa
Additional bands at : 160 kDa,260 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Immunoprecipitation - HERC2 antibody (ab85832)
Immunoprecipitation/ Western BlotFor IP: Lane 1: ab85832 at 3µg/mg whole cell lysate.Lane 2: Control IgG.ab85832 at 1µg/ml for WBWhole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.Chemiluminescence with an exposure time of 30 seconds.
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