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Western blot - HERC2 antibody (ab85832)
(enlarge)
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Immunoprecipitation - HERC2 antibody (ab85832)
(enlarge)
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Product Name
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HERC2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HERC2
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Immunogen
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Synthetic peptide, corresponding to a region within amino acids 4784-4834 of Human HERC2 (NP_004658.3)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 527 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from HeLa cells.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
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Relevance
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HERC2 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HERC2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HERC2 antibody images, references, products related to ab85832 and other tools.
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HERC2 antibody images:
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 Western blot - HERC2 antibody (ab85832)
All lanes : HERC2 antibody (ab85832) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
developed using the ECL technique
Predicted band size : 527 kDa
Observed band size : 527 kDa
Additional bands at : 160 kDa,260 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
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Immunoprecipitation - HERC2 antibody (ab85832)
Immunoprecipitation/ Western BlotFor IP:
Lane 1: ab85832 at 3µg/mg whole cell lysate.Lane 2: Control IgG.ab85832 at 1µg/ml for WBWhole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.Chemiluminescence with an exposure time of 30 seconds.
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Search PubMed (MEDLINE) for references to HERC2
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HERC2 antibody - more information
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Customer reviews (feedback) regarding HERC2 antibody |
Customer FAQs regarding HERC2 antibody |
Protocols for HERC2 antibody |
Price and availability of products related to HERC2 antibody |
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HERC2 antibody - related products:
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HERC2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HERC2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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