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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Overview
Product name
Anti-HERC2 antibody
Description
Rabbit polyclonal to HERC2
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region within amino acids 4784-4834 of Human HERC2 (NP_004658.3)
Positive control
Whole cell lysate from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Hect E3 Ligase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Predicted molecular weight: 527 kDa.
IP: Use at 2-5 µg/mg of lysate.
Target
Relevance
HERC2 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Alternative names
- HECT domain and RCC1 like domain containing protein 2 antibody
- Hect domain and RLD 2 antibody
- jdf2 antibody
see all
Database links
- Entrez Gene: 8924 Human
- Omim: 605837 Human
- SwissProt: O95714 Human
Anti-HERC2 antibody images:
Western blot - HERC2 antibody (ab85832)
All lanes : Anti-HERC2 antibody (ab85832) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
developed using the ECL technique
Predicted band size : 527 kDa
Observed band size : 527 kDa
Additional bands at : 160 kDa,260 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Immunoprecipitation - HERC2 antibody (ab85832)
Immunoprecipitation/ Western BlotFor IP:Lane 1: ab85832 at 3µg/mg whole cell lysate.Lane 2: Control IgG.ab85832 at 1µg/ml for WBWhole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.Chemiluminescence with an exposure time of 30 seconds.
References for Anti-HERC2 antibody (ab85832)
ab85832 has not yet been referenced specifically in any publications.
Publishing research using ab85832? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"