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Anti-HES5 antibody (ab25374)

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Overview

Product name

Anti-HES5 antibody
See all HES5 products (3) ...

Description

Rabbit polyclonal to HES5

Tested applications

ELISA, WB, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide: SLHQDYSEGYSWC, corresponding to amino acids 83-95 of Mouse HES5

SLHQDYSEGY SWC

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 40% Glycerol, PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Neurology process >> Notch Pathway

  • Immunohistochemistry (Frozen sections) - HES5 antibody (ab25374)Immunohistochemistry (Frozen sections) - HES5 antibody (ab25374) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
IHC-Fr: 1/200 - 1/1000. This product has not been tested with paraffin sections.
WB: 1/200 - 1/1000. Predicted molecular weight: 19 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional repressor of genes that require a bHLH protein for their transcription.

Tissue specificity

Expressed in fetal heart and brain tumors.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.

Domain

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ5TA89 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • bHLHb38 antibody
  • Class B basic helix-loop-helix protein 38 antibody
  • Hairy and enhancer of split 5 antibody
  • HES 5 antibody
  • Hes5 antibody
  • HES5 protein antibody
  • HES5_HUMAN antibody
  • MGI:104876 antibody
  • Transcription factor HES-5 antibody
see all

Database links

Anti-HES5 antibody images:

  Immunohistochemistry (Frozen sections) - HES5 antibody (ab25374)

Immunohistochemistry (Frozen sections) - HES5 antibody (ab25374)

ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr.  The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes.  A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes.  HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.

References for Anti-HES5 antibody (ab25374)

This product has been referenced in:

  • Marchong MNet al. Cdh11 acts as a tumor suppressor in a murine retinoblastoma model by facilitating tumor cell death. PLoS Genet 6:e1000923 (2010).Read more (PubMed: 20421947) »
  • Takanaga Het al. Gli2 Is a Novel Regulator of Sox2 Expression in Telencephalic Neuroepithelial Cells. Stem Cells 27:165-174 (2009). IHC-Fr; Mouse.Read more (PubMed: 18927476) »

See all 3 publications for this product

Publishing research using ab25374? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"