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Immunohistochemistry (Frozen sections) - HES5 antibody (ab25374)
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Product Name
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HES5 antibody
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See all HES5 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HES5
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Immunogen
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Synthetic peptide: SLHQDYSEGYSWC, corresponding to amino acids 83-95 of Mouse HES5
BLAST 'SLHQDYSEGYSWC' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
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ELISA, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-Fr: 1/200 - 1/1000. This product has not been tested with paraffin sections.
WB: 1/200 - 1/1000. Predicted molecular weight: 19 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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The transcription factor Hes5 (Hairy and enhancer of split 5) is a basic helix-loop-helix (bHLH) transcriptional repressor that inhibits neural differentiation. The Hes gene products Hes1, Hes3 and Hes5, are essential for the maintenance of neural stem cells and to ensure that neuronal cells are made both in correct numbers and full diversity. Hes proteins antagonise activator bHLH proteins, such Mash1, Math and Neurogenin, which promote neuronal fate determination and induce expression of Notch ligands such as Delta. These ligands upregulate Hes1 and Hes5 gene expression in neighbouring cells, ensuring that these are maintained in an undifferentiated state.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 40% Glycerol, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HES5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HES5 antibody images, references, products related to ab25374 and other tools.
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HES5 antibody images:
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 Immunohistochemistry (Frozen sections) - HES5 antibody (ab25374)
ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr. The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes. A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes. HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.
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References for HES5 antibody (ab25374)
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This product has been used in: (two most recent references)
Takanaga H et al. Gli2 Is a Novel Regulator of Sox2 Expression in Telencephalic Neuroepithelial Cells. Stem Cells 27:165-174 (2009). IHC-Fr; Mouse. PubMed: 18927476
Ma QH et al. A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis. Nat Cell Biol 10:283-94 (2008). WB; Mouse. PubMed: 18278038
If you publish research using ab25374 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HES5
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HES5 antibody - more information
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Customer reviews (feedback) regarding HES5 antibody |
Customer FAQs regarding HES5 antibody |
Protocols for HES5 antibody |
Price and availability of products related to HES5 antibody |
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HES5 antibody - related products:
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HES5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HES5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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