Anti-HES5 antibody (ab65077)
- Product nameAnti-HES5 antibodySee all HES5 primary antibodies ...
- DescriptionRabbit polyclonal to HES5
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse HES5.
(Peptide available as ab85813.)
- Positive controlThis antibody gave a positive signal in both Mouse and Rat Liver Tissue Lysates.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab65077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 18 kDa).Can be blocked with HES5 peptide (ab85813).|
- FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription.
- Tissue specificityExpressed in fetal heart and brain tumors.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
- DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
- Cellular localizationNucleus.
- bHLHb38 antibodyClass B basic helix-loop-helix protein 38 antibodyHairy and enhancer of split 5 antibody
- HES 5 antibodyHes5 antibodyHES5 protein antibodyHES5_HUMAN antibodyMGI:104876 antibodyTranscription factor HES-5 antibody
Anti-HES5 antibody images
All lanes : Anti-HES5 antibody (ab65077) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HES5 antibody (ab65077)
ab65077 has not yet been referenced specifically in any publications.