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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-HES5 antibody
See all HES5 products (3) ...
Description
Rabbit polyclonal to HES5
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse HES5.
(Peptide available as ab85813.)
Positive control
This antibody gave a positive signal in both Mouse and Rat Liver Tissue Lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - HES5 antibody (ab65077)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 40 kDa (predicted molecular weight: 18 kDa).
Target
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription.
Tissue specificity
Expressed in fetal heart and brain tumors.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ5TA89
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
see all
Database links
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 137268 Mouse
- Unigene: 22422 Rat
Anti-HES5 antibody images:
Western blot - HES5 antibody (ab65077)
All lanes : Anti-HES5 antibody (ab65077) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
The 40 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to HES5.
References for Anti-HES5 antibody (ab65077)
ab65077 has not yet been referenced specifically in any publications.
Publishing research using ab65077? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"