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Western blot - HES5 antibody (ab65077)
(enlarge)
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Product Name
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HES5 antibody
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See all HES5 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HES5
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse HES5.
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Reacts with
(species key)
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Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in both Mouse and Rat Liver Tissue Lysates.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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The transcription factor Hes5 (Hairy and enhancer of split 5) is a basic helix-loop-helix (bHLH) transcriptional repressor that inhibits neural differentiation. The Hes gene products Hes1, Hes3 and Hes5, are essential for the maintenance of neural stem cells and to ensure that neuronal cells are made both in correct numbers and full diversity. Hes proteins antagonise activator bHLH proteins, such Mash1, Math and Neurogenin, which promote neuronal fate determination and induce expression of Notch ligands such as Delta. These ligands upregulate Hes1 and Hes5 gene expression in neighbouring cells, ensuring that these are maintained in an undifferentiated state.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HES5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HES5 antibody images, references, products related to ab65077 and other tools.
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HES5 antibody images:
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 Western blot - HES5 antibody (ab65077)
All lanes : HES5 antibody (ab65077) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
The 40 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to HES5.
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Search PubMed (MEDLINE) for references to HES5
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HES5 antibody - more information
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Customer reviews (feedback) regarding HES5 antibody |
Customer FAQs regarding HES5 antibody |
Protocols for HES5 antibody |
Price and availability of products related to HES5 antibody |
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HES5 antibody - related products:
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HES5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HES5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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