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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab65077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 18 kDa).Can be blocked with HES5 peptide (ab85813).

Target

  • FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificityExpressed in fetal heart and brain tumors.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q5TA89 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        bHLHb38 antibodyClass B basic helix-loop-helix protein 38 antibodyHairy and enhancer of split 5 antibody
        HES 5 antibodyHes5 antibodyHES5 protein antibodyHES5_HUMAN antibodyMGI:104876 antibodyTranscription factor HES-5 antibody
      see all

    Anti-HES5 antibody images

    • Western blot - HES5 antibody (ab65077)
      Western blot - HES5 antibody (ab65077)
      All lanes : Anti-HES5 antibody (ab65077) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Liver (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 18 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-HES5 antibody (ab65077)

    ab65077 has not yet been referenced specifically in any publications.

    Publishing research using ab65077 ? Please let us know so that we can cite the reference in this datasheet

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    Why do different Hes5 antibodies show different molecular weights? The expected MW is 18 kDa, what would cause it to run higher than 40 kDa?

    Thank you very much for your previous phone call and for your patience while we have looked into your enquiry.

    At this time, we don't have a conclusive explanation for the different band sizes of this protein, and it may be due to different prot...

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