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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-HEXIM1 antibody - ChIP Grade
See all HEXIM1 products (5) ...
Description
Rabbit polyclonal to HEXIM1 - ChIP Grade
Tested applications
ChIP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human HEXIM1.
(Peptide available as ab26978.)
Positive control
This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa Jurkat A431 HEK293 (Data not shown) MCF7 SHSY-5Y Mouse Whole Cell Lysate MEF1 NIH 3T3 (Data not shown) Mouse Tissue Lysates Kidney (Data not shown) Testis (Data not shown) Ovary (Data not shown) Rat Tissue Lysate Kidney (Data not shown)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
ChIP: Use at an assay dependent concentration (see Abreview)
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
Tissue specificity
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
Sequence similarities
Belongs to the HEXIM family.
Domain
The coiled-coil domain mediates oligomerization.
Cellular localization
Nucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear.
Target information above from: UniProt accessionO94992
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cardiac lineage protein 1 antibody
- CLP 1 antibody
- CLP1 antibody
see all
Database links
- Entrez Gene: 10614 Human
- Entrez Gene: 192231 Mouse
- Entrez Gene: 498008 Rat
- Omim: 607328 Human
- SwissProt: O94992 Human
- SwissProt: Q8R409 Mouse
- SwissProt: Q5M9G1 Rat
- Unigene: 15299 Human
see all
Anti-HEXIM1 antibody - ChIP Grade images:
Western blot - HEXIM1 antibody - ChIP Grade (ab25388)
All lanes : Anti-HEXIM1 antibody - ChIP Grade (ab25388) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 41 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
ab25388 recognizes a band at approximately 54 kDa that corresponds in size to that seen for HEXIM1. Although it has a predicted molecular weight of 41 kDa, it has been shown to migrate at a larger size of about 54-60 kDa (see Byers et al., J Biol Chem. 2005 Apr 22;280(16):16360-7 and Schulte et al., J. Biol. Chem., Vol. 280, (26): 24968-24977).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEXIM1 antibody - ChIP Grade (ab25388)
Image courtesy of Human Protein Atlas
ab25388 staining HEXIM1 in human kidney. Paraffin embedded human kidney tissue was incubated with ab25388 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab25388 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
References for Anti-HEXIM1 antibody - ChIP Grade (ab25388)
This product has been referenced in:
- Benedikt Aet al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB, IP; Human.Read more (PubMed: 21030982) »
- Lin Cet al. AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia. Mol Cell 37:429-37 (2010).Read more (PubMed: 20159561) »
See all 4 publications for this product
Publishing research using ab25388? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"