Anti-HEXO antibody [1B3] (ab119413)

Overview

• Product nameAnti-HEXO antibody [1B3]See all HEXO primary antibodies ...
• Description
  Mouse monoclonal [1B3] to HEXO
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Rat, Human, Monkey
  
• Immunogen

  Recombinant full length Human HEXO (NP_699163) produced in HEK293T cell.

• Positive controlHEK293T cells transfected with the pCMV6-ENTRY HEXO; cells lysates from HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells; Human Kidney tissue; Human endometrium tissue; Carcinoma of Human bladder tissue; Human tonsil.
• General notesDilute with PBS when in use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein A purified
• Purification notesPurified from mouse ascites fluids by affinity chromatography
• Clonality Monoclonal
• Clone number1B3
• IsotypeIgG2b
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Scaffold Proteins

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA / Nucleotides

Applications

Target

• FunctionRNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
• Sequence similaritiesContains 1 exonuclease domain.
  Contains 1 SAP domain.
• DomainThe SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.
• Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus.

Target information above from: UniProt accession Q8IV48 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 90459 Human
  • Entrez Gene: 361159 Rat
  • Omim: 608739 Human
  • SwissProt: Q8IV48 Human
  • SwissProt: Q5FVR4 Rat
  • Unigene: 20000 Human
  • Unigene: 17469 Rat

• Alternative names
  3' 5' exonuclease ERI 1 antibody3' 5' exonuclease ERI1 antibody3' EXO antibody

  3' exoribonuclease antibody3' HEXO antibody3' histone mRNA exonuclease 1 antibody3''-5'' exonuclease eri1 antibody3''-5'' exoribonuclease 1 antibody3'EXO antibody3'HEXO antibodyERI 1 antibodyEri 1 homolog antibodyEri-1 homolog antibodyERI1 antibodyEri1 homolog antibodyERI1_HUMAN antibodyHEXO antibodyHistone mRNA 3' end specific exonuclease antibodyHistone mRNA 3' end specific exoribonuclease antibodyHistone mRNA 3''-end-specific exoribonuclease antibodyHistone mRNA 3''-exonuclease 1 antibodyMGC35395 antibodyProtein 3''hExo antibodyTHEX 1 antibodyTHEX1 antibodyThree prime histone mRNA exonuclease 1 antibody

  see all

Anti-HEXO antibody [1B3] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXO antibody [1B3] (ab119413)
  Immunohistochemical staining of paraffin-embedded Human tonsil using 1/150 ab119413.
• 

  Western blot - Anti-HEXO antibody [1B3] (ab119413)
  All lanes : Anti-HEXO antibody [1B3] (ab119413) at 1/500 dilution
  
  Lane 1 : HEK293T cells transfected with the pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY HEXO cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 40 kDa
  
• 

  Western blot - Anti-HEXO antibody [1B3] (ab119413)
  All lanes : Anti-HEXO antibody [1B3] (ab119413) at 1/500 dilution
  
  Lane 1 : HepG2 cells lysate
  Lane 2 : HeLa cells lysate
  Lane 3 : SVT2 cells lysate
  Lane 4 : A549 cells lysate
  Lane 5 : COS7 cells lysate
  Lane 6 : Jurkat cells lysate
  Lane 7 : MDCK cells lysate
  Lane 8 : PC12 cells lysate
  Lane 9 : MCF7 cells lysate
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 40 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXO antibody [1B3] (ab119413)
  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using 1/150 ab119413.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXO antibody [1B3] (ab119413)
  Immunohistochemical staining of paraffin-embedded Human endometrium tissue using 1/150 ab119413.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXO antibody [1B3] (ab119413)
  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using 1/150 ab119413