Recombinant full length Human HEXO (NP_699163) produced in HEK293T cell.
HEK293T cells transfected with the pCMV6-ENTRY HEXO; cells lysates from HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells; Human Kidney tissue; Human endometrium tissue; Carcinoma of Human bladder tissue; Human tonsil.
General notesDilute with PBS when in use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
FunctionRNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Sequence similaritiesContains 1 exonuclease domain. Contains 1 SAP domain.
DomainThe SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.