Overview

  • Product nameAnti-HEXO antibody [1B3]
    See all HEXO primary antibodies
  • Description
    Mouse monoclonal [1B3] to HEXO
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human, Monkey
  • Immunogen

    Recombinant full length Human HEXO (NP_699163) produced in HEK293T cell.

  • Positive control
    • HEK293T cells transfected with the pCMV6-ENTRY HEXO; cells lysates from HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells; Human Kidney tissue; Human endometrium tissue; Carcinoma of Human bladder tissue; Human tonsil.
  • General notesDilute with PBS when in use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-P 1/150.

Target

  • FunctionRNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
  • Sequence similaritiesContains 1 exonuclease domain.
    Contains 1 SAP domain.
  • DomainThe SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.
  • Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3' 5' exonuclease ERI 1 antibody
    • 3' 5' exonuclease ERI1 antibody
    • 3' EXO antibody
    • 3' exoribonuclease antibody
    • 3' HEXO antibody
    • 3' histone mRNA exonuclease 1 antibody
    • 3''-5'' exonuclease eri1 antibody
    • 3''-5'' exoribonuclease 1 antibody
    • 3'EXO antibody
    • 3'HEXO antibody
    • ERI 1 antibody
    • Eri 1 homolog antibody
    • Eri-1 homolog antibody
    • ERI1 antibody
    • Eri1 homolog antibody
    • ERI1_HUMAN antibody
    • HEXO antibody
    • Histone mRNA 3' end specific exonuclease antibody
    • Histone mRNA 3' end specific exoribonuclease antibody
    • Histone mRNA 3''-end-specific exoribonuclease antibody
    • Histone mRNA 3''-exonuclease 1 antibody
    • MGC35395 antibody
    • Protein 3''hExo antibody
    • THEX 1 antibody
    • THEX1 antibody
    • Three prime histone mRNA exonuclease 1 antibody
    see all

Anti-HEXO antibody [1B3] images

  • Immunohistochemical staining of paraffin-embedded Human tonsil using 1/150 ab119413.
  • All lanes : Anti-HEXO antibody [1B3] (ab119413) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with the pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY HEXO cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206777)
  • All lanes : Anti-HEXO antibody [1B3] (ab119413) at 1/500 dilution

    Lane 1 : HepG2 cells lysate
    Lane 2 : HeLa cells lysate
    Lane 3 : SVT2 cells lysate
    Lane 4 : A549 cells lysate
    Lane 5 : COS7 cells lysate
    Lane 6 : Jurkat cells lysate
    Lane 7 : MDCK cells lysate
    Lane 8 : PC12 cells lysate
    Lane 9 : MCF7 cells lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206777)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using 1/150 ab119413.
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue using 1/150 ab119413.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using 1/150 ab119413

References for Anti-HEXO antibody [1B3] (ab119413)

ab119413 has not yet been referenced specifically in any publications.

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