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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614)
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Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614)
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Western blot - HEY1 antibody (ab22614)
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Product Name
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HEY1 antibody
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See all HEY1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HEY1
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Immunogen
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Synthetic peptide derived from a region between residues 207-261 of Human HEY1.
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Reacts with
(species key)
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Hu
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Specificity
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ab22614 detects a band at ~34kDa (corresponding to the mw of human Hey1) in Western Blot analysis of fetal lung tissue and has been used in immunohistochemical applications to detect human alveolar cells and renal tubule epithelial cells.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50,000 - 100,000 as second antibody. Predicted molecular weight: 34 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal lung lysate, Human lung or Human kidney tissue.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. Hey1 functions as a corepressor, providing a mechanism of cross-talk between Notch and androgen signaling pathways. It is excluded from the nucleus in most human prostate cancers.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HEY1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HEY1 antibody images, references, products related to ab22614 and other tools.
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HEY1 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614)
Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X. Alveolar cells are positively labelled (indicated with arrows).
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Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614)
Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X. Epithelial cells of renal tubules are positively labelled (indicated with arrows).
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Western blot - HEY1 antibody (ab22614)
Lane 1 : HEY1 antibody (ab22614) at 5 µg/ml
Lane 2 : HEY1 antibody (ab22614) at 2.5 µg/ml
Lane 3 : HEY1 antibody (ab22614) at 1.25 µg/ml
Lane 1 : Cell lysate prepared from human fetal lung cells
Lane 2 : Cell lysate prepared from human fetal lung cells
Lane 3 : Cell lysate prepared from human fetal lung cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 34 kDa
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References for HEY1 antibody (ab22614)
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This product has been used in:
Elagib KE et al. Jun blockade of erythropoiesis: role for repression of GATA-1 by HERP2. Mol Cell Biol 24:7779-94 (2004). PubMed: 15314183
If you publish research using ab22614 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HEY1
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HEY1 antibody - more information
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Customer reviews (feedback) regarding HEY1 antibody |
Customer FAQs regarding HEY1 antibody |
Protocols for HEY1 antibody |
Price and availability of products related to HEY1 antibody |
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HEY1 antibody - related products:
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HEY1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HEY1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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