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Anti-HEY1 antibody (ab22614)

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Overview

Product name

Anti-HEY1 antibody
See all HEY1 products (4) ...

Description

Rabbit polyclonal to HEY1

Specificity

ab22614 detects a band at ~34kDa (corresponding to the mw of human Hey1) in Western Blot analysis of fetal lung tissue and has been used in immunohistochemical applications to detect human alveolar cells and renal tubule epithelial cells.

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat, Dog

Immunogen

Synthetic peptide derived from a region between residues 207-261 of Human HEY1.

Positive control

Fetal lung lysate, Human lung or Human kidney tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614)Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614) image (enlarge)

  • Western blot - HEY1 antibody (ab22614)Western blot - HEY1 antibody (ab22614) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab22614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 34 kDa.( 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody.)

  • ShowHide2 Images

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 4 - 8 µg/ml.

Target

Function

Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

Tissue specificity

Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.

Sequence similarities

Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9Y5J3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Basic helix loop helix protein OAF1 antibody
  • bHLHb31 antibody
  • Cardiovascular helix loop helix factor 2 antibody
  • Cardiovascular helix loop-helix factor 2 antibody
  • Cardiovascular helix-loop-helix factor 2 antibody
  • CHF 2 antibody
  • CHF-2 antibody
  • CHF2 antibody
  • Class B basic helix-loop-helix protein 31 antibody
  • Hairy and enhancer of split related protein 1 antibody
  • Hairy and enhancer of split-related protein 1 antibody
  • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
  • Hairy related transcription factor 1 antibody
  • Hairy-related transcription factor 1 antibody
  • Hairy/enhancer of split related with YRPW motif 1 antibody
  • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
  • HERP 2 antibody
  • HERP2 antibody
  • HES related repressor protein 1 antibody
  • HES-related repressor protein 1 antibody
  • HES-related repressor protein 2 antibody
  • HESR 1 antibody
  • HESR-1 antibody
  • HESR1 antibody
  • HEY 1 antibody
  • hey1 antibody
  • HEY1_HUMAN antibody
  • hHRT1 antibody
  • HRT 1 antibody
  • HRT-1 antibody
  • HRT1 antibody
  • MGC1274 antibody
  • OAF 1 antibody
  • OAF1 antibody
see all

Anti-HEY1 antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614)

Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml.  Magnification 400X.

Alveolar cells are positively labelled (indicated with arrows).

  Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614)

Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614)

Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.

Epithelial cells of renal tubules are positively labelled (indicated with arrows).

  Western blot - HEY1 antibody (ab22614)

Western blot - HEY1 antibody (ab22614)

Lane 1 : Anti-HEY1 antibody (ab22614) at 5 µg/ml
Lane 2 : Anti-HEY1 antibody (ab22614) at 2.5 µg/ml
Lane 3 : Anti-HEY1 antibody (ab22614) at 1.25 µg/ml

Lane 1 : Cell lysate prepared from human fetal lung cells
Lane 2 : Cell lysate prepared from human fetal lung cells
Lane 3 : Cell lysate prepared from human fetal lung cells

Lysates/proteins at 25 µg per lane.


Predicted band size : 34 kDa

References for Anti-HEY1 antibody (ab22614)

This product has been referenced in:

  • Belyea BCet al. Inhibition of the notch-hey1 axis blocks embryonal rhabdomyosarcoma tumorigenesis. Clin Cancer Res 17:7324-36 (2011). WB, IHC-P; Human.Read more (PubMed: 21948088) »
  • Hanlon Let al. Notch1 functions as a tumor suppressor in a model of K-ras-induced pancreatic ductal adenocarcinoma. Cancer Res 70:4280-6 (2010). IHC-P; Mouse.Read more (PubMed: 20484026) »

See all 3 publications for this product

Publishing research using ab22614? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"