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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-HEY1 antibody
See all HEY1 products (4) ...
Description
Rabbit polyclonal to HEY1
Specificity
ab22614 detects a band at ~34kDa (corresponding to the mw of human Hey1) in Western Blot analysis of fetal lung tissue and has been used in immunohistochemical applications to detect human alveolar cells and renal tubule epithelial cells.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog
Immunogen
Synthetic peptide derived from a region between residues 207-261 of Human HEY1.
Positive control
Fetal lung lysate, Human lung or Human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 34 kDa.( 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody.)
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Tissue specificity
Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
Sequence similarities
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y5J3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop helix factor 2 antibody
see all
Database links
- Entrez Gene: 23462 Human
- Entrez Gene: 15213 Mouse
- Entrez Gene: 155437 Rat
- Omim: 602953 Human
- SwissProt: Q9Y5J3 Human
- SwissProt: Q9WV93 Mouse
- Unigene: 234434 Human
- Unigene: 29581 Mouse
see all
Anti-HEY1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HEY1 antibody (ab22614)
Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.
Alveolar cells are positively labelled (indicated with arrows).
Immunohistochemistry (Paraffin-embedded sections) - HEY1 antibody (ab22614)
Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.
Epithelial cells of renal tubules are positively labelled (indicated with arrows).
Western blot - HEY1 antibody (ab22614)
Lane 1 : Anti-HEY1 antibody (ab22614) at 5 µg/ml
Lane 2 : Anti-HEY1 antibody (ab22614) at 2.5 µg/ml
Lane 3 : Anti-HEY1 antibody (ab22614) at 1.25 µg/ml
Lane 1 : Cell lysate prepared from human fetal lung cells
Lane 2 : Cell lysate prepared from human fetal lung cells
Lane 3 : Cell lysate prepared from human fetal lung cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 34 kDa
References for Anti-HEY1 antibody (ab22614)
This product has been referenced in:
- Belyea BCet al. Inhibition of the notch-hey1 axis blocks embryonal rhabdomyosarcoma tumorigenesis. Clin Cancer Res 17:7324-36 (2011). WB, IHC-P; Human.Read more (PubMed: 21948088) »
- Hanlon Let al. Notch1 functions as a tumor suppressor in a model of K-ras-induced pancreatic ductal adenocarcinoma. Cancer Res 70:4280-6 (2010). IHC-P; Mouse.Read more (PubMed: 20484026) »
See all 3 publications for this product
Publishing research using ab22614? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"