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Western blot - HEY2 / Hry2 antibody (ab70133)
(enlarge)
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Product Name
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HEY2 antibody
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See all HEY2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HEY2
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Immunogen
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Synthetic peptide derived from an internal sequence of human HEY2 / Hry2
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab70133 detects endogenous levels of total HEY2 / Hry2 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K562 cell extracts.
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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The Hey family of bHLH transcription factors are direct targets of Notch signaling. HEY2 (gridlock) is an important determinant of mammalian heart development, loss of which in mouse leads to cardiac defects with high postnatal lethality. Mouse Hey1 knockouts have no apparent phenotypic defect but the combined loss of Hey1 and Hey2 results in embryonic death after embryonic day 9.5 (E9.5) with a global lack of vascular remodeling and massive hemorrhage. Similar defects are observed in Jagged1 and Notch1 knockout mice.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab70133 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HEY2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HEY2 antibody images, references, products related to ab70133 and other tools.
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HEY2 antibody images:
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 Western blot - HEY2 / Hry2 antibody (ab70133)
All lanes : HEY2 antibody (ab70133) at 1/500 dilution
Lane 1 : K562 cell extracts
Lane 2 : K562 cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 36 kDa
Observed band size : 36 kDa
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Search PubMed (MEDLINE) for references to HEY2
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HEY2 antibody - more information
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Customer reviews (feedback) regarding HEY2 antibody |
Customer FAQs regarding HEY2 antibody |
Protocols for HEY2 antibody |
Price and availability of products related to HEY2 antibody |
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HEY2 antibody - related products:
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HEY2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HEY2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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