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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-HEY2 antibody
See all HEY2 products (4) ...
Description
Rabbit polyclonal to HEY2
Specificity
ab70133 detects endogenous levels of total HEY2 / Hry2 protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from an internal sequence of human HEY2 / Hry2
Positive control
K562 cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70133 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - HEY2 / Hry2 antibody (ab70133)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Sequence similarities
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBP5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic helix-loop-helix factor 1 antibody
- Basic helix-loop-helix protein antibody
- bHLH antibody
see all
Database links
- Entrez Gene: 23493 Human
- Entrez Gene: 15214 Mouse
- Omim: 604674 Human
- SwissProt: Q9UBP5 Human
- SwissProt: Q9QUS4 Mouse
- Unigene: 144287 Human
- Unigene: 103573 Mouse
Anti-HEY2 antibody images:
Western blot - HEY2 / Hry2 antibody (ab70133)
All lanes : Anti-HEY2 antibody (ab70133) at 1/500 dilution
Lane 1 : K562 cell extracts
Lane 2 : K562 cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 36 kDa
Observed band size : 36 kDa
References for Anti-HEY2 antibody (ab70133)
ab70133 has not yet been referenced specifically in any publications.
Publishing research using ab70133? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"