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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-HEY2 antibody
See all HEY2 products (4) ...
Description
Rabbit polyclonal to HEY2
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse HEY2 / Hry2.
(Peptide available as ab38053.)
Positive control
ab25404 gave a positive signal in Mouse Heart and Skeletal Muscle Tissue Lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - HEY2 / Hry2 antibody (ab25404)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
Target
Function
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Sequence similarities
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBP5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic helix-loop-helix factor 1 antibody
- Basic helix-loop-helix protein antibody
- bHLH antibody
see all
Database links
- Entrez Gene: 23493 Human
- Entrez Gene: 15214 Mouse
- Omim: 604674 Human
- SwissProt: Q9UBP5 Human
- SwissProt: Q9QUS4 Mouse
- Unigene: 144287 Human
- Unigene: 103573 Mouse
Anti-HEY2 antibody images:
Western blot - HEY2 / Hry2 antibody (ab25404)
All lanes : Anti-HEY2 antibody (ab25404) at 2 µg/ml
Lane 1 : Heart (Mouse)Tissue Lysate
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HEY2 antibody (ab25404)
This product has been referenced in:
- Zanotti Set al. Reciprocal Regulation of Notch and Nuclear Factor of Activated T-cells (NFAT) c1 Transactivation in Osteoblasts. J Biol Chem 286:4576-88 (2011). WB; Mouse.Read more (PubMed: 21131365) »
See 1 publication for this product
Publishing research using ab25404? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"