Anti-HFE antibody [3F1] (ab125156)

Overview

• Product nameAnti-HFE antibody [3F1]See all HFE primary antibodies ...
• Description
  Mouse monoclonal [3F1] to HFE
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified recombinant fragment of Human HFE, expressed in E. Coli.

  Run BLAST with Run BLAST with
• Positive controlHFE-hIgGFc transfected HEK293 cell lysate. HepG2 cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium azide
  Constituent: 99% Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number3F1
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Cofactors, Vitamins / minerals
  • Vitamins / minerals

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Erythrocytic Lineage

  • Signal Transduction
  • Metabolism
  • Vitamins / Minerals

Applications

Target

• FunctionBinds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
• Tissue specificityExpressed in all tissues tested except brain.
• Involvement in diseaseDefects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
  Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
  Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
• Sequence similaritiesBelongs to the MHC class I family.
  Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q30201 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3077 Human
  • Omim: 613609 Human
  • SwissProt: Q30201 Human
  • Unigene: 233325 Human

• Alternative names
  dJ221C16.10.1 antibodyHemochromatosis antibodyHemochromatosis protein antibody

  Hereditary hemochromatosis protein antibodyHereditary hemochromatosis protein HLA H antibodyHFE 1 antibodyHFE antibodyHFE_HUMAN antibodyHFE1 antibodyHH antibodyHigh Fe antibodyHLA H antibodyHLA-H antibodyHLAH antibodyMGC:150812 antibodyMGC10379 antibodyMGC103790 antibodyMHC class I like protein HFE antibodyMVCD7 antibodyTFQTL2 antibody

  see all

Anti-HFE antibody [3F1] images

• 

  Western blot - Anti-HFE antibody [3F1] (ab125156)
  Anti-HFE antibody [3F1] (ab125156) at 1/500 dilution + HFE recombinant protein
  
  Predicted band size : 40 kDa
  
• 

  Western blot - Anti-HFE antibody [3F1] (ab125156)
  All lanes : Anti-HFE antibody [3F1] (ab125156) at 1/500 dilution
  
  Lane 1 : HEK293 cell lysate
  Lane 2 : HFE-hIgGFc transfected HEK293 cell lysate
  
  
  Predicted band size : 40 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-HFE antibody [3F1] (ab125156)
  ab125156, at 1/200 dilution, staining HFE in HepG2 cells by immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.