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Products:Signal Transduction >> Metabolism >> Vitamins / Minerals
Overview
Product name
Anti-HFE antibody
See all HFE products (4) ...
Description
Mouse monoclonal to HFE
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: SHTLQVILGC EMQEDNSTEG YWKYGYDGQD HLEFCPDTLD WRAAEPRAWP TKLEWERHKI RARQNRAYLE RDCPAQLQQL LELGRGVLDQ Q, corresponding to amino acids 115-206 of Human HFE
SHTLQVILGC EMQEDNSTEG YWKYGYDGQD HLEFCPDTLD WRAAEPRAWP TKLEWERHKI RARQNRAYLE RDCPAQLQQL LELGRGVLDQ Q
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Signal Transduction >> Metabolism >> Vitamins / Minerals
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
Tissue specificity
Expressed in all tissues tested except brain.
Involvement in disease
Defects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ30201
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dJ221C16.10.1 antibody
- Hemochromatosis antibody
- Hemochromatosis protein antibody
see all
Database links
- Entrez Gene: 3077 Human
- Omim: 235200 Human
- SwissProt: Q30201 Human
- Unigene: 233325 Human
Anti-HFE antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-HFE antibody (ab55182)
ICC/IF image of HFE staining (ab55182) in human blood film. The sections were incubated in 0.3% Triton X with 0.1M PBS to permeabilise the cells and in 10% serum for 30 minutes at RT to block non-specific protein-protein interactions. The sections were then incubated with ab55182 (1:500) for 4 hrs at RT, followed by Alexa 568 conjugated secondary antibody. Red staining of the cell membrane was observed. Macrophages were stained using a CD68 primary and Alexa Fluor 488 conjugated secondary antibody. The nucleus was visualised using Hoechst.
This image was taken from an abreview submitted by Ruma Raha-Chowdhury.
Western blot - HFE antibody (ab55182)
Western blot against tagged recombinant protein immunogen using ab55182 HFE antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
Immunocytochemistry/ Immunofluorescence-HFE antibody(ab55182)
ab55182 at 10 ug/ml staining HFE in human Hela cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-HFE antibody (ab55182)
ab55182 has not yet been referenced specifically in any publications.
Publishing research using ab55182? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"