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Western blot - HFE antibody (ab55182)
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Immunocytochemistry/ Immunofluorescence-HFE antibody(ab55182)
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Product Name
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HFE antibody
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See all HFE antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to HFE
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Immunogen
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Recombinant fragment: SHTLQVILGC EMQEDNSTEG YWKYGYDGQD HLEFCPDTLD WRAAEPRAWP TKLEWERHKI RARQNRAYLE RDCPAQLQQL LELGRGVLDQ Q, corresponding to amino acids 115-206 of Human HFE
BLAST 'SHTLQVILGC EMQEDNSTEG YWKYGYDGQD HLEFCPDTLD WRAAEPRAWP TKLEWERHKI RARQNRAYLE RDCPAQLQQL LELGRGVLDQ ' with  or 
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Reacts with
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Hu
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; Single-pass type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Signal Transduction >> Metabolism >> Vitamins / Minerals
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Relevance
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Hemochromatosis protein (HFE) is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that HFE functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HFE antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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HFE antibody images:
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 Western blot - HFE antibody (ab55182)
Western blot against tagged recombinant protein immunogen using ab55182 HFE antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
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Immunocytochemistry/ Immunofluorescence-HFE antibody(ab55182)
ab55182 at 10 ug/ml staining HFE in human Hela cells by Immunocytochemistry/ Immunofluorescence.
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Search PubMed (MEDLINE) for references to HFE
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HFE antibody - more information
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Customer reviews (feedback) regarding HFE antibody |
Customer FAQs regarding HFE antibody |
Protocols for HFE antibody |
Price and availability of products related to HFE antibody |
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HFE antibody - other tools:
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Contact Abcam with a Technical Enquiry about HFE antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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