Anti-HGD antibody (ab231968)
Key features and details
- Rabbit polyclonal to HGD
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-HGD antibody
See all HGD primary antibodies -
Description
Rabbit polyclonal to HGD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human, Orangutan -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse HGD aa 150 to the C-terminus. (Expressed in E.coli)
Database link: O09173 -
Positive control
- WB: Recombinant mouse HGD protein; Mouse and rat liver lysates. IHC-P: Mouse kidney and liver tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231968 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Tissue specificity
Highest expression in the prostate, small intestine, colon, kidney and liver. -
Pathway
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. -
Involvement in disease
Defects in HGD are the cause of alkaptonuria (AKU) [MIM:203500]. AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis. -
Sequence similarities
Belongs to the homogentisate dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 3081 Human
- Entrez Gene: 15233 Mouse
- Entrez Gene: 100171592 Orangutan
- Entrez Gene: 360719 Rat
- Omim: 607474 Human
- SwissProt: Q93099 Human
- SwissProt: O09173 Mouse
- SwissProt: Q5RF05 Orangutan
see all -
Alternative names
- 2-dioxygenase antibody
- AKU antibody
- FLJ94126 antibody
see all
Images
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Paraffin-embedded mouse liver tissue stained for HGD using ab231968 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse kidney tissue stained for HGD using ab231968 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-HGD antibody (ab231968) at 3 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa -
Anti-HGD antibody (ab231968) at 3 µg/ml + Recombinant mouse HGD protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231968 has not yet been referenced specifically in any publications.