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Overview

  • Product nameHGF Human ELISA KitSee all HGF kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 81% - 104%
    Tissue Extracts 95.5 80% - 103%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100535 Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF cell lysate and tissue lysate. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • FunctionHGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.
  • Involvement in diseaseDefects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.

    • Target information above from: UniProt accession P14210 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        DFNB39F TCFFibroblast derived tumor cytotoxic factor
        Hepapoietin AHepatocyte growth factorHepatocyte growth factor (hepapoietin A scatter factorHepatocyte growth factor beta chainHepatopoietin-AHgfHGF_HUMANHGFBHPTALung fibroblast derived mitogenScatter factorSF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100535 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      HGF Human ELISA Kit images

      • ELISA - HGF Human ELISA Kit (ab100535)
        ELISA - HGF Human ELISA Kit (ab100535)

      Protocols

      References for HGF Human ELISA Kit (ab100535)

      ab100535 has not yet been referenced specifically in any publications.

      Publishing research using ab100535 ? Please let us know so that we can cite the reference in this datasheet

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      Inquiry: I would like to know of this product if it recognizes the single chain and/or heterodimer variant of hepatocyte growth factor. Kind regards,


      This kit is expected to detect both the single unprocessed chain and the heterodimer HGF protein.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"