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Overview

  • Product nameHGF Human ELISA KitSee all HGF kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 3 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    > 98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.35 89% - 108%
    Serum 108.28 96% - 120%
    Plasma 107.56 95% - 119%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100534 HGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF in serum, plasma (EDTA and citrate), cell culture supernatants and urine. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • FunctionHGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.
  • Involvement in diseaseDefects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39) [MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.

    • Target information above from: UniProt accession P14210 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        DFNB39F TCFFibroblast derived tumor cytotoxic factor
        Hepapoietin AHepatocyte growth factorHepatocyte growth factor (hepapoietin A scatter factorHepatocyte growth factor beta chainHepatopoietin-AHgfHGF_HUMANHGFBHPTALung fibroblast derived mitogenScatter factorSF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100534 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      HGF Human ELISA Kit images

      • ELISA - HGF Human ELISA Kit (ab100534)
        ELISA - HGF Human ELISA Kit (ab100534)

      Protocols

      References for HGF Human ELISA Kit (ab100534)

      ab100534 has not yet been referenced specifically in any publications.

      Publishing research using ab100534 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100534 Submit a Question for ab100534

      Displaying 1 - 10 of 14 results for Abreviews and Q&A

      10
      Votes: Highest First

      Dear Support Team,

      I’m currently investigating a new HGF ELISA and came across a few questions regarding your product (HGF Human ELISA Kit (ab100534)).

      · Does this kit detect total HGF - the single chain precursor of...

      Read More

      Thank you for your inquiry. I have answered your questions below:

      Does this kit detect total HGF - the single chain precursor of HGF (scHGF) and the activated heterodimer as well?

      The exact form of HGF recognized has not been tested, how...

      Read More

      Dear xxxx

      Thank you very much for your kind help.
      The customer has also requested If possible, to see the precision, range and accuracy data for the kit, as well as data concerning the stability of the HGF standard.
      It would be very helpf...

      Read More

      I am sorry for the delay in getting back to you. I now have further data to share with you from the HGF Human ELISA Kit (ab100534).

      Please find attached an excel spreadsheet. In the first sheet there is the data collected from two subsequent assa...

      Read More

      Dear xxxx

      Thank you very much for your assistance.
      The lot used is GR95296-1 but they used standard from both lots because they had spare from the first kit.
      One of their questions is if it's ok to use a properly stored standard from one ...

      Read More

      I am sorry for the delay in getting back to you. I now have further information to share with you in relation the variation seem by your customer when using the HGF Human ELISA Kit (ab100534).

      The source of this kti ahve had a look at the results...

      Read More

      Dear Tech support
      One of our customers has ordered ab100534 twice.
      The customer has some spare standard from the first kit left and wonders if it's recommended to use it with the second kit in order to save some standard for future work with the...

      Read More

      Thank you for contacting us.

      The reconstituted standard is stable for ˜1 week if stored frozen (-20°C - -80°C). Please ensure avoiding multiple freeze – thaw cycles. We do provide 2 vials of the standard recombinant protein to mini...

      Read More

      Dear xxxx
      Thank you for your reply...

      The order details are:
      xxxxxx

      Warm regards,

      Thank you for confirming that. xxxx is currently not in the office but I can help with this case.

      I have now added the replacement kit to the order number xxxxxx. Please be aware that the kit may be moved from this order before shipping to be co...

      Read More

      The customer reallyappreciates your detailed and well thought of answer, however, the viscosity issue of the buffer still remains.
      According to the customer it really makes a difference in the duplicates because it's hard to maintain a constant volum...

      Read More

      Thank you for your reply.

      It can't be that the buffer has the same consistency as glycerol!As we do not have separatecomponents, I would like to offer your customer a FOCR instead.

      Please let me know if this is acceptable.

      One of our clients has ordered ab100534 and wishes to get some advise regarding the procedure and results:

      Here are the customer questions:

      "Endothel cells from two donors overexpressing the gene ANG-1 were plated and grown in C...

      Read More

      Thank you for sending in the data. The standard curve does still contain strong linearity when including the higher concentrated standards, 222.2 pg/ml and 666.7 pg/ml. The highest standard (2000 pg/ml) did give a reading that affects the linearity down t...

      Read More

      There is no problem with the assay itself, my question is to clarify the values of the standards for the assay. When the standard is reconstituted with Assay Diluent B as prescribed in the protocol, the protocol states that a 2000pg/ml standard is created...

      Read More

      Thank you for contacting us.

      260 uL of Assay Diluent A or 1x Assay Diluent B should be added to all of the vials except the first one. The first vial should contain 390 uL of the Standard that was prepared in 600 uL of Assay Diluent A or B. That...

      Read More

      Abcam's TDS for ab100534 states "gentle shaking" for incubation, could you please specify the rpm or settings one should use with it's shaker?

      It is difficult to recommend a specific speed for a "gentle shaking". The plate should just bemoving to get a homogenous solution and not too fast to avoid a spillage.I hope this helps.

      Will this kit detect only activated HGF or will it detect total HGF?

      Thank you for contacting us.

      Both mouse and human HGF ELISA kits will detect total HGF.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"