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Please advise suggested positive control for this ab in WB |
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ANSWER: |
Thank you for your enquiry. The following publication lists a few samples that can be used as a positive control for this HGFA Inhibitor 1 antibody. Distribution of Hepatocyte Growth Factor Activator Inhibitor Type 1 (HAI-1) in Human Tissues: Cellular Surface Localization of HAI-1 in Simple Columnar Epithelium and Its Modulated Expression in Injured and Regenerative Tissues. Journal of Histochemistry and Cytochemistry, Vol. 47, 673-682, May 1999 http://www.jhc.org/cgi/content/full/47/5/673 Western blot: MKN-28 cells, gastric mucosa lysate IHC: pancreas, prostate, endocervix of uterus, colon mucosal tissue Products available at Abcam: ab4611 - Pancreas (human) normal tissue slides ab4361 - Prostate (human) normal tissue slides I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-HGFA Inhibitor 1 antibody - Aminoterminal end (ab28330) at 1/1000 dilution
Lane 1 : HGFA Inhibitor 1 at 0.05 µg
Lane 2 : HGFA Inhibitor 1 at 0.01 µg
Lane 3 : HGFA Inhibitor 1 at 0.001 µg
Predicted band size : 59 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible cleavage fragment).
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