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Products:Immunology >> Secreted Molecules >> Other secreted molecules
Overview
Product name
Anti-HGFA Inhibitor 1 antibody - Cytoplasmic domain
See all HGFA Inhibitor 1 products (6) ...
Description
Rabbit polyclonal to HGFA Inhibitor 1 - Cytoplasmic domain
Specificity
This antibody is specific for HGFA Inhibitor 1 and HAI-1B (a splice variant).
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the cytoplasmic domain of human HGFA Inhibitor 1 sequence.
(Peptide available as ab41270.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> HAIs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Immunology >> Secreted Molecules >> Other secreted molecules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects bands of approximately 58kDa and 40kDa (predicted molecular weight: 58 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).
Sequence similarities
Contains 2 BPTI/Kunitz inhibitor domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MANSC domain.
Domain
This inhibitor contains two inhibitory domains.
Cellular localization
Secreted.
Target information above from: UniProt accessionO43278
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HAI 1 antibody
- HAI antibody
- HAI-1 antibody
see all
Database links
- Entrez Gene: 6692 Human
- Omim: 605123 Human
- SwissProt: O43278 Human
- Unigene: 233950 Human
Anti-HGFA Inhibitor 1 antibody - Cytoplasmic domain images:
Western blot - HGFA Inhibitor 1 antibody - Cytoplasmic domain (ab28333)
Anti-HGFA Inhibitor 1 antibody - Cytoplasmic domain (ab28333) at 1/1000 dilution + SF at 0.05 µg
Predicted band size : 58 kDa
Observed band size : 58,95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa (possible cleavage fragment).
Immunocytochemistry/ Immunofluorescence - HGFA Inhibitor 1 antibody - Cytoplasmic domain (ab28333)
ICC/IF image of ab28333 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28333, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-HGFA Inhibitor 1 antibody - Cytoplasmic domain (ab28333)
ab28333 has not yet been referenced specifically in any publications.
Publishing research using ab28333? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"