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Anti-HGS antibody (ab56468)

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Overview

Product name

Anti-HGS antibody
See all HGS products (7) ...

Description

Mouse monoclonal to HGS

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment: KLEIMRQKKQ EYLEVQRQLA IQRLQEQEKE RQMRLEQQKQ TVQMRAQMPA FPLPYAQLQA MPAAGGVLYQ PSGPASFPST FSPAGSVEGS PMHGVYMSQP , corresponding to amino acids 513-612 of Human HGS

KLEIMRQKKQ EYLEVQRQLA IQRLQEQEKE RQMRLEQQKQ TVQMRAQMPA FPLPYAQLQA MPAAGGVLYQ PSGPASFPST FSPAGSVEGS PMHGVYMSQP

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG1

Light chain type

kappa

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin

  • Western blot - HGS antibody (ab56468)Western blot - HGS antibody (ab56468) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab56468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/400 (PMID 19684575).
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 86 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Tissue specificity

Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.

Sequence similarities

Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.

Domain

Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

Post-translational
modifications

Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH.

Cellular localization

Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.

Target information above from: UniProt accessionO14964 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
  • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
  • HGNC:4897 antibody
  • HGS antibody
  • HGS_HUMAN antibody
  • Hrs antibody
  • Human growth factor regulated tyrosine kinase substrate antibody
  • Protein pp110 antibody
  • ZFYVE8 antibody
see all

Database links

Anti-HGS antibody images:

  Western blot - HGS antibody (ab56468)

Western blot - HGS antibody (ab56468)

HGS antibody (ab56468) at 1ug/lane + K-562 cell lysate at 25ug/lane.

References for Anti-HGS antibody (ab56468)

This product has been referenced in:

  • Gibbings DJet al. Multivesicular bodies associate with components of miRNA effector complexes and modulate miRNA activity. Nat Cell Biol 11:1143-9 (2009). WB, ICC/IF; Human.Read more (PubMed: 19684575) »
  • Gorla Let al. Proteomics study of medullary thyroid carcinomas expressing RET germ-line mutations: identification of new signaling elements. Mol Carcinog 48:220-31 (2009).Read more (PubMed: 18756447) »

See all 2 publications for this product

Publishing research using ab56468? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"