Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734)

Overview

• Product nameAnti-HIF Prolyl Hydroxylases antibody [EPR3664(2)]See all HIF Prolyl Hydroxylases primary antibodies ...
• Description
  Rabbit monoclonal [EPR3664(2)] to HIF Prolyl Hydroxylases
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues in Human HIF Prolyl Hydroxylases.

• Positive control293T, U87-MG, HeLa, HepG2, and SH-SY5Y cell lysates, Human brain tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3664(2)
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Hypoxia
  • Hydroxylases

  • Cardiovascular
  • Hypoxia
  • Prolyl Hydroxylase

  • Epigenetics and Nuclear Signaling
  • Cardiovascular/Immune
  • Hypoxia
  • Prolyl hydroxylase

Applications

Target

• FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
• Tissue specificityWidely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
• Sequence similaritiesContains 2 EF-hand domains.
  Contains 1 Fe2OG dioxygenase domain.
• Post-translational
  modificationsGlycosylated.
• Cellular localizationEndoplasmic reticulum membrane.

Target information above from: UniProt accession Q9NXG6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 54681 Human
  • SwissProt: Q9NXG6 Human
  • Unigene: 654944 Human

• Alternative names
  EGLN4 antibodyFLJ20262 antibodyHIF prolyl hydroxylase PH4 antibody

  HIF-PH4 antibodyHIF-prolyl hydroxylase 4 antibodyHIFPH4 antibodyHPH-4 antibodyHypoxia inducible factor prolyl 4 hydroxylase antibodyHypoxia inducible factor prolyl hydroxylase antibodyHypoxia-inducible factor prolyl hydroxylase 4 antibodyP4H TM antibodyP4H with transmembrane domain antibodyP4H-TM antibodyP4htm antibodyP4HTM_HUMAN antibodyPH 4 antibodyPH4 antibodyPHD4 antibodyProline 4 hydroxylase antibodyProlyl 4 hydroxylase transmembrane (endoplasmic reticulum) antibodyProlyl hydroxlase domain containing 4 antibodyTransmembrane prolyl 4-hydroxylase antibody

  see all

Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] images

• 

  Western blot - HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734)
  All lanes : Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734) at 1/1000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : U87-MG cell lysate
  Lane 3 : HeLa cell lysate
  Lane 4 : HepG2 cell lysate
  Lane 5 : SH-SY5Y cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 57 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734)
  Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109734 at a dilution of 1/100.