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Products:Cardiovascular >> Hypoxia >> HIF

Anti-HIF1 alpha (Hydroxy P402) antibody (ab72775)

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Overview

Product name

Anti-HIF1 alpha (Hydroxy P402) antibody
See all HIF1 alpha products (22) ...

Description

Rabbit polyclonal to HIF1 alpha (Hydroxy P402)

Specificity

This antibody reacts specifically with HIF1 alpha (Hydroxy P402). No crossreactivity was shown with non modified peptide.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

a wide range of other species

Immunogen

A 15 residue synthetic peptide derived from human HIF1 alpha containing hydroxyproline at 402 position.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: 1/200 - 1/1000.Predi...Read more →

    WB: 1/200 - 1/1000.Predicted molecular weight: 93 kDa.

Target

Function

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.

Tissue specificity

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.

Domain

Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).

Post-translational
modifications

In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

Cellular localization

Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.

Target information above from: UniProt accessionQ16665 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Basic helix loop helix PAS protein MOP1 antibody
  • ARNT interacting protein antibody
  • ARNT-interacting protein antibody
  • Basic-helix-loop-helix-PAS protein MOP1 antibody
  • bHLHe78 antibody
  • Class E basic helix-loop-helix protein 78 antibody
  • HIF 1A antibody
  • HIF 1alpha antibody
  • HIF-1-alpha antibody
  • HIF1 A antibody
  • HIF1 Alpha antibody
  • HIF1-alpha antibody
  • HIF1A antibody
  • HIF1A_HUMAN antibody
  • Hypoxia inducible factor 1 alpha antibody
  • Hypoxia inducible factor 1 alpha isoform I.3 antibody
  • Hypoxia inducible factor 1 alpha subunit antibody
  • Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
  • Hypoxia-inducible factor 1-alpha antibody
  • Member of PAS protein 1 antibody
  • Member of PAS superfamily 1 antibody
  • Member of the PAS Superfamily 1 antibody
  • MOP 1 antibody
  • MOP1 antibody
  • PAS domain-containing protein 8 antibody
  • PASD 8 antibody
  • PASD8 antibody
see all

Database links

References for Anti-HIF1 alpha (Hydroxy P402) antibody (ab72775)

This product has been referenced in:

  • Wiley Met al. Toxoplasma gondii activates hypoxia-inducible factor (HIF) by stabilizing the HIF-1alpha subunit via type I activin-like receptor kinase receptor signaling. J Biol Chem 285:26852-60 (2010). WB; Human.Read more (PubMed: 20581113) »

See 1 publication for this product

Publishing research using ab72775? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"