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Thank you very much for your interest. Yes, delivery adress is still the same as you have in your email. |
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ANSWER: |
The order 1085220 has been created for ab818. You will be receiving the product soon. |
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I am emailing regarding order #1077900. |
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ANSWER: |
Thank you for your message. |
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Ab1 and the replacement antibody ab463 did not work in Western blot. |
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ANSWER: |
Thank you for your call today. |
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Thanks for your kindly reply; this customer has not yet to decide the free of replacement, |
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ANSWER: |
Thank you for your note. |
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Thanks for your kindly reply, the information of this product as follow: |
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ANSWER: |
Further to our previous correspondence, 1003954 is not the PON (Purchase Order Number) but the Abcam Order Number (20 Dec 2011). The PON was AB-100100. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-HIF1 alpha antibody [H1alpha67] - ChIP Grade (ab1) at 5 µg/ml
Lane 1 : Hela - Vehicle only (Negative Control) Whole Cell Lysate
Lane 2 : Hela - DFO treated (0.5mM, 24h) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 92 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Anti-HIF1 alpha antibody [H1alpha67] - ChIP Grade (ab1) at 1/400 dilution + Human Cell lysate - whole cell (human lung adenocarcinoma cell line ADLC-5M2) treated for 16 hours with 100 micromolar deferoxamine (DFO)
at 20 µg
Performed under reducing conditions.
Predicted band size : 92 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
PVDF membrane was used and blocked for 16 hours in 5% milk.
This image is taken from an Abreview submitted by Mike Campa, no further information is known about this image
ab1 at 1/200 dilution staining HIF1 alpha in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized in 0.5% Trition X-100 and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/500 dilution.
This Image is courtesy of an anonymous Abreview
ab1 staining HIF1 alpha in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeablized with 0.2% Triton-X100 before blocking with 2% BSA for 30 minutes at 20°C. The sample was incubated with primary antibody (1/200) for 9 hours at 4°C. An Alexa Fluor®555-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution. DAPI was used to stain the cell nuclei (blue).
This image is courtesy of an anonymous Abreview
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