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Western blot - HIF1 alpha antibody [H1alpha67] (ab1)
![Western blot - HIF1 alpha antibody [H1alpha67] (ab1)](/ps/datasheet/images/ab1_2.jpg)
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Immunocytochemistry/ Immunofluorescence - HIF1 alpha antibody [H1alpha67] (ab1)
![Immunocytochemistry/ Immunofluorescence - HIF1 alpha antibody [H1alpha67] (ab1)](/ps/datasheet/images/0/ab1/HIF1-alpha-Primary-antibodies-ab1-6.jpg)
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Product Name
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HIF1 alpha antibody [H1alpha67]
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See all HIF1 alpha antibodies (24)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [H1alpha67] to HIF1 alpha
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Immunogen
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Fusion protein: DVMLPSPN E KLQNINLAM SPLPTAETPK PLRSSADPA L NQEVALKL EP NPESLEL SFT MPQIQD QTPS PSDGS TRQSS PEPN SPSEYC FYV DSDMV, corresponding to amino acids 432-528 of Human HIF1 alpha
BLAST 'DVMLPSPN E KLQNINLAM SPLPTAETPK PLRSSADPA L NQEVALKL EP NPESLEL SFT MPQIQD QTPS PSDGS TRQSS PEPN SPSEYC FYV ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Frt, Mk, Pig, Rb, Shp
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/1300.
IHC-P: 1/25 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/400 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC-P on glioblastoma multiforme and also WB on nuclear extracts of a hypoxically or CoCl2 induced cell line such as MCF-7, PC12, 293, or MEF.
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Cellular localization
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Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF
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Relevance
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Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF1 alpha) has been identified as a bHLH-PAS family member that is instrumental in the oxygen-dependent regulation of these genes. HIF1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerises with the aryl hydrocarbon nuclear receptor translocator ARNT (also referred to as HIF1 beta). Heterodimerisation promotes efficient DNA binding.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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H1alpha67
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Isotype
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IgG2b
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Protein G purified from mouse ascites.
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Form
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Liquid
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Concentration
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1.300 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HIF1 alpha antibody [H1alpha67] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HIF1 alpha antibody [H1alpha67] images, references, products related to ab1 and other tools.
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HIF1 alpha antibody [H1alpha67] images:
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 Western blot - HIF1 alpha antibody [H1alpha67] (ab1)
HIF1 alpha antibody [H1alpha67] (ab1) at 1/400 dilution + Human Cell lysate - whole cell (human lung adenocarcinoma cell line ADLC-5M2) treated for 16 hours with 100 micromolar deferoxamine (DFO)
at 20 µg
Performed under reducing conditions.
Predicted band size : 92 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
PVDF membrane was used and blocked for 16 hours in 5% milk. This image is taken from an Abreview submitted by Mike Campa, no further information is known about this image
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Immunocytochemistry/ Immunofluorescence - HIF1 alpha antibody [H1alpha67] (ab1)
ab1 at 1/200 dilution staining HIF1 alpha in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized in 0.5% Trition X-100 and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/500 dilution. This Image is courtesy of an anonymous Abreview
See Abreview
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References for HIF1 alpha antibody [H1alpha67] (ab1)
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This product has been used in: (two most recent references)
Fisher CD et al. Hepatic cytochrome P450 enzyme alterations in humans with progressive stages of nonalcoholic fatty liver disease. Drug Metab Dispos 37:2087-94 (2009). PubMed: 19651758
Bailey JM et al. Sonic hedgehog paracrine signaling regulates metastasis and lymphangiogenesis in pancreatic cancer. Oncogene 28:3513-25 (2009). WB. PubMed: 19633682
See all 29 publication references for this product.
If you publish research using ab1 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HIF1 alpha
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HIF1 alpha antibody [H1alpha67] - more information
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HIF1 alpha antibody [H1alpha67] - related products:
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HIF1 alpha antibody [H1alpha67] - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - HIF1 alpha antibody [H1alpha67] (ab1)](/index.html?datasheet=1#ab1_2.jpg)
![Immunocytochemistry/ Immunofluorescence - HIF1 alpha antibody [H1alpha67] (ab1)](/index.html?datasheet=1#HIF1-alpha-Primary-antibodies-ab1-6.jpg)
