HIF1 alpha antibody [H1alpha67-sup] (ab463)

Product Name 

HIF1 alpha antibody [H1alpha67-sup]

Product type 

Primary antibodies

Description 

Mouse monoclonal [H1alpha67-sup] to HIF1 alpha

Immunogen 

Fusion protein, corresponding to amino acids 432-528 of Human HIF 1 alpha.

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Frt, Pig, Shp

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/1000. Antigen retrieval is not essential but may optimise staining. See Zong et al 2001 reference (or protocol at http://www.ihcworld.com/_protocols/antibody_protocols/hif_1alpha_novus.htm).
Optimal antigen retrieval method: Briefly, pre-heat steamer or water bath with staining dish containing 10 mM citrate buffer pH 6.0 until temperature reaches 95-100°C. Immerse slides in the staining dish and place the lid loosely on the staining dish. Incubate for 45 minutes and turn off the steamer or water bath. Place the staining dish at room temperature and allow the slides to cool for 20 minutes.
ICC/IF: See recommended protocol below.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.


Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Glioblastoma multiforme.

Cellular localization 

Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Cardiovascular/Immune >> Hypoxia >> HIF
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Cardiovascular >> Hypoxia >> HIF

Relevance 

Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF1 alpha) has been identified as a bHLH-PAS family member that is instrumental in the oxygen-dependent regulation of these genes. HIF1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerises with the aryl hydrocarbon nuclear receptor translocator ARNT (also referred to as HIF1 beta). Heterodimerisation promotes efficient DNA binding.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

H1alpha67-sup

Isotype 

IgG2b

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: PBS; FBS (trace).

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Protein G purified from cell culture supernatant.

Form 

Liquid

Concentration 

Batch dependent within range: 2.00 - 2.90 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C. Do not freeze.

References for HIF1 alpha antibody [H1alpha67-sup] (ab463)

  This product has been used in: (two most recent references)

Ryou MG et al. Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass. Am J Physiol Heart Circ Physiol 297:H1914-22 (2009). WB; Pig. PubMed: 19767525

Amarilio R et al. HIF1alpha regulation of Sox9 is necessary to maintain differentiation of hypoxic prechondrogenic cells during early skeletogenesis. Development 134:3917-28 (2007). PubMed: 17913788

See all 22 publication references for this product.


If you publish research using ab463 please let us know so that we can cite the reference on this datasheet.

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